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  1. Link

    goat-mouse-igg-hl-pe-preadsorbed-ab97041.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (PE) preadsorbed (ab97041)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

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Find out more.

Key features and details

  • Goat Anti-Mouse IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Flow Cyt

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (PE) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • Tested applications

    Suitable for: Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Conjugation

    PE. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to PE.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Phycoerythrin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50 - 1/200.
Notes
Flow Cyt
1/50 - 1/200.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab97041? Please let us know so that we can cite the reference in this datasheet.

ab97041 has been referenced in 5 publications.

  • Lee ACK  et al. Mitochondrial Fuel Dependence on Glutamine Drives Chemo-Resistance in the Cancer Stem Cells of Hepatocellular Carcinoma. Int J Mol Sci 22:N/A (2021). PubMed: 33805044
  • Yamane M  et al. Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Extensively Differentiate to Tyrosine-Hydroxylase-Expressing Dopamine-Secreting Neurons. Cells 10:N/A (2021). PubMed: 33920157
  • St-Cyr D  et al. Identification and optimization of molecular glue compounds that inhibit a noncovalent E2 enzyme-ubiquitin complex. Sci Adv 7:eabi5797 (2021). PubMed: 34705497
  • Minocha E  et al. Amniotic fluid stem cells ameliorate cisplatin-induced acute renal failure through induction of autophagy and inhibition of apoptosis. Stem Cell Res Ther 10:370 (2019). PubMed: 31801607
  • Takao M  et al. Flow cytometric quantitation of EpCAM-positive extracellular vesicles by immunomagnetic separation and phospholipid staining method. Genes Cells 23:963-973 (2018). PubMed: 30220098

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

I intend to use this secondary antibody for flow cytometry analysis. I would like to know the absorption and the emission wavelength of the flourophore attached to this antibody.

Read More

Abcam community

Verified customer

Asked on Feb 06 2013

Answer

Thank you for contacting us.

The absorption and emission spectrum as well as the maximum wavelength can be found here:

https://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf

The maximum excitation wavelengths are 496 and 566 nm, the maximum emission wavelength are 576 nm, and the excitation laser line is 488 nm.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 06 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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