Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

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Overview

  • Product name

    Goat Anti-Mouse IgG H&L (TRITC)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details

  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. (PubMed:15659422)
ELISA 1/10000 - 1/50000.

Images

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
    Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.

References

This product has been referenced in:

  • Yu F  et al. Programmed death ligand-1, tumor infiltrating lymphocytes and HLA expression in Chinese extrahepatic cholangiocarcinoma patients: Possible immunotherapy implications. Biosci Trends 13:58-69 (2019). Read more (PubMed: 30773525) »
  • Wang Z  et al. A Phage Lysin Fused to a Cell-Penetrating Peptide Kills Intracellular Methicillin-Resistant Staphylococcus aureus in Keratinocytes and Has Potential as a Treatment for Skin Infections in Mice. Appl Environ Microbiol 84:N/A (2018). Read more (PubMed: 29625989) »
See all 20 Publications for this product

Publishing research using ab6786? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I bought the primary antibody (Primary antibody (mouse anti CK3): https://www.abcam.com/Cytokeratin-3-antibody-AE5-ab77869.html) and secondary antibody (goat anti-mouse 1)https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-FITC-pre-adsorbed-ab97039.html
2) https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6786.html) from you.

And I used rabbit cornea as the control and I tried several times for the positive control and negative control. But the results were weird.










All the slides which stained with FITC were positive (for negative control also showed positive staining)

All the slides which stained with TRITC were negative (for positive control also showed negative staining)



And I attach the immunostaining fotos and the protocol what I used for the immunostaing in the attachment. Could you please help me check if the antibodies what I chosen are not proper or the protocol what I used is not proper?? If it is, please send me a proper protocol for the immunostaining ! Thank you very much for your help!

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Answer

Thank you for contacting Abcam.

I will be happy to help you further in this matter, but I just have a few more questions for you:

1 - Were you staining tissue sections or a monolayer of cultured cells?

2 - If you were staining sections, how were they prepared (PfA fixation & paraffin embedded, MeOH etc)?

3 - If these samples were paraffin embedded, what type of antigen retrieval did you use on them?

4 - Have you used either of the secondary antibodies successfully with other primary antibodies?

I look forward to your reply and helping to resolve these issues.

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Question

I would just like to know if your secondary antibody ab6786 (goat polyclonal to mouse IgG H&L conjugated to Rhodamine) would be suitable in conjunction with antibody ab8329 (EBNA 1 antibody, mouse monoclonal IgG2b). It is not listed in your list of recommended secondaries for this antibody, but I cannot see from the data sheet why it would not work, Please advise,

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Answer

Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

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