Overview

  • Product name
    Goat Anti-Mouse IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. (PubMed:15659422)
ELISA 1/10000 - 1/50000.

Images

  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.

References

This product has been referenced in:
  • Li L  et al. Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development. PLoS Negl Trop Dis 12:e0006309 (2018). Read more (PubMed: 29538424) »
  • Laura B  et al. Epigenetic control of defense genes following MeJA-induced priming in rice (O. sativa). J Plant Physiol 228:166-177 (2018). Read more (PubMed: 29936261) »
See all 15 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

I will be happy to help you further in this matter, but I just have a few more questions for you:

1 - Were you staining tissue sections or a monolayer of cultured cells?

2 - If you were staining sections, how were they prepared (PfA fixation & paraffin embedded, MeOH etc)?

3 - If these samples were paraffin embedded, what type of antigen retrieval did you use on them?

4 - Have you used either of the secondary antibodies successfully with other primary antibodies?

I look forward to your reply and helping to resolve these issues.

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Answer

Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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