Key features and details
- Goat Anti-Mouse IgG1 heavy chain
- Host species: Goat
- Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WB
Product nameGoat Anti-Mouse IgG1 heavy chain
See all IgG1 secondary antibodies
SpecificityThe anti IgG1 only reacts with IgG1 by IEP and gel diffusion analysis when tested against each of the mouse IgG subclasses at 1 mg/ml.
Tested applicationsSuitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details
Goats were immunized with highly purified mouse IgG1 in Freunds adjuvant
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe resulting antiserum was sequentially absorbed with IgG2a, IgG2b and IgG3 immunoabsorbants until it was IgG1 specific. The anti IgG1 was then immunoaffinity purified and tested for monospecificity.
Our Abpromise guarantee covers the use of ab9165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Immunodiffusion||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9165 has been referenced in 2 publications.
- Fradkin AH et al. UV photodegradation of murine growth hormone: Chemical analysis and immunogenicity consequences. Eur J Pharm Biopharm N/A:N/A (2014). PubMed: 24758742
- Fradkin AH et al. Glass particles as an adjuvant: a model for adverse immunogenicity of therapeutic proteins. J Pharm Sci 100:4953-64 (2011). PubMed: 21721003