Overview

  • Product name

    Goat Anti-Mouse IgG1 heavy chain
    See all IgG1 secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%.
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICCmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/30000. (Coating ELISA: 1/100 - 1/500, Primary ELISA: 1/1000-1/30000)
WB 1/1000 - 1/30000. (Chemiluminecent: 1/1000 - 1/30000, Colorimetric: 1/1000 - 1/10000)
IHC-P 1/200 - 1/2000.
ICC Use at an assay dependent dilution.

References

ab97236 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

Please check the following datasheets as answer to your first question;

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

The antibodies to your second question is

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-ab97240.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-ab97239.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-pre-adsorbed-ab98689.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-ab97236.html

For searching the right secondary antibody; please use the secondary search tool on our website.

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

The band size seems to be correct. This product has a predicted weight of 39kDa although you band seems a bit smaller this is common when using in real world settings as compared to predictions. Post translational modifications are a common cause of this as well as the inherent inaccuracies of protein marker standards. These are often labeled as 'apparent' size because they may be off by 3-30kDa.
In regards to further data and publications, to our knowledge this product has not been published with. We always include any information that we may have regarding publications, reviews or any information that we about the antibody on our website.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting Abcam and thank you for sending the details of your protocol as well as the image. These have been very helpful.

After reviewing your protocol, I would like to suggest a few tips which may help reduce the upper dark areas and increase the band quality for Rhodopsin.
First I would recommend changing your blocking procedures. It may be best for this product if your initial blocking and primary antibody incubation are performed using 3% BSA in TBST. This should be followed by use of a casein based commercial block for incubating your secondary antibody. We have seen that this may dramatically reduce background in western blot. We do carry this type of casein based blocking reagent, ab64234 (https://www.abcam.com/Background-Reducing-Buffer-ab64234-support.html).

Second, you may wish to reduce the concentration of secondary antibody that you use. This product has a large range 1:2000-1:20,000 against which it has been used effectively.

Finally I would suggest increasing the concentration of detergent in your TBST, if you currently use 0.1% Tween20 use 0.25% Tween20 instead.

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Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this recommended product was not correct for your assay and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number I have also attached a pdf file with our recommended protocol for stripping western blots. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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