Goat Anti-Mouse IgG1 heavy chain (Allophycocyanin/Cy7 ®) preadsorbed (ab130785)

Overview

  • Product name

    Goat Anti-Mouse IgG1 heavy chain (Allophycocyanin/Cy7 ®) preadsorbed
    See all IgG1 secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Allophycocyanin/Cy7®), pre-adsorbed
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    ab130785 reacts with the heavy chain of Mouse IgG1 as demonstrated by ELISA, FLISA and/or Flow Cytometry. Goat Anti-Mouse IgG1 heavy chain (Allophycocyanin/Cy7 ®) preadsorbed shows minimal cross reactivity with Human immunoglobulins.

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, ELISAmore details
  • Minimal
    cross-reactivity


    Human more details
  • Immunogen

    Mouse IgG1 paraproteins

  • Conjugation

    Allophycocyanin/Cy7 ®. Ex: 650nm, Em: 774nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: PBS, Sucrose
  • Concentration information loading...
  • Purification notes

    ab130785 was purified by affinity chromatography on Mouse IgG1 covalently linked to agarose. ab130785 was cross absorbed with Mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled Human sera and purified Human paraproteins.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF Use 0.1µg for 106 cells.
ELISA Use at an assay dependent concentration.

References

ab130785 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The concentration of this lot is GR91861-1 is 0.85mg/ml. An appropriate isotype control would be ab91353, Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. This product is shipped with a concentration of 1mg/ml. https://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html We recommend use of a pre-absorbed secondary antibody in Flow Cytometry. We have a number of pre-absorbed anti-Mouse IgG1 antibodies which are conjugated to fluorescent molecules for use in Flow. Choice of the appropriate marker will depend upon your experimental set up and flow cytometer. I have complied a short list for you below. Secondary Antibodies: AB98692 (FITC conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-pre-adsorbed-ab98692.html AB136127 (Cy5 conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-gamma-chain-Cy5--pre-adsorbed-ab136127.html AB130785 (AP/Cy7 conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-AllophycocyaninCy7-pre-adsorbed-ab130785.html AB130786 (AP conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-Allophycocyanin-pre-adsorbed-ab130786.html

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