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Goat Anti-Mouse IgG1 heavy chain (Biotin) preadsorbed (ab98691)

Submit a review Q&A (1)References (7)

Key features and details

  • Goat Anti-Mouse IgG1 heavy chain (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, ELISA, WB

Overview

  • Product name

    Goat Anti-Mouse IgG1 heavy chain (Biotin) preadsorbed
    See all IgG1 secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab98691 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/250 - 1/2500.
ICC/IF
1/100 - 1/500.
ELISA
1/1000 - 1/10000. as primary
WB
1/10000 - 1/200000. by chemiluminescent detection
Notes
IHC-P
1/250 - 1/2500.
ICC/IF
1/100 - 1/500.
ELISA
1/1000 - 1/10000. as primary
WB
1/10000 - 1/200000. by chemiluminescent detection

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (7)

Publishing research using ab98691? Please let us know so that we can cite the reference in this datasheet.

ab98691 has been referenced in 7 publications.

  • Katsur M  et al. Exosomes from neuronal stem cells may protect the heart from ischaemia/reperfusion injury via JAK1/2 and gp130. J Cell Mol Med 25:4455-4465 (2021). PubMed: 33797200
  • Lee SS  et al. A novel 3D histotypic cartilage construct engineered by supercritical carbon dioxide decellularized porcine nasal cartilage graft and chondrocytes exhibited chondrogenic capability in vitro. Int J Med Sci 18:2217-2227 (2021). PubMed: 33859530
  • Takov K  et al. Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential. Basic Res Cardiol 115:26 (2020). PubMed: 32146560
  • Sun P  et al. Clusterin Deficiency Predisposes C57BL/6j Mice to Cationic Bovine Serum Albumin-Induced Glomerular Inflammation. J Inflamm Res 13:969-983 (2020). PubMed: 33262633
  • Takov K  et al. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles 8:1560809 (2019). PubMed: 30651940
  • Takov K  et al. Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes. J Extracell Vesicles 6:1388731 (2017). PubMed: 29184625
  • Nokia MS  et al. Physical exercise increases adult hippocampal neurogenesis in male rats provided it is aerobic and sustained. J Physiol 594:1855-73 (2016). PubMed: 26844666

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

LOT NUMBER GR53220 ORDER NUMBER 975372 DESCRIPTION OF THE PROBLEM No staining SAMPLE mouse: lung, pancreas, CT26-Tumor, LPS-liver PRIMARY ANTIBODY 1:25 and 1:50 in PBS incubation overnight at roomtemperature DETECTION METHOD Steaptavidin-POD, 1:50, 10 min at RT POSITIVE AND NEGATIVE CONTROLS USED negative control: sections without primary antibody ANTIBODY STORAGE CONDITIONS -20°C FIXATION OF SAMPLE 4% Formalin ANTIGEN RETRIEVAL Citrat-buffer pH 6.0, microwave 600 Watt, 10 min PERMEABILIZATION STEP none BLOCKING CONDITIONS 3% H2O2 in Methanol for 10 min 3% Goatserum in PBS 30 min Endogenous Avidin + Biotin Blocking System (ab3387) SECONDARY ANTIBODY goat-anti-mouse biotin (ab98696, 1:100, 45 min at RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? dilution of primary antibody ADDITIONAL NOTES we used pancreas as postitive control because of the pictures in the datasheet

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Answer

Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen. Es tut mir leid, dass Sie Probleme mit unserem VEGF-Antikörper ab3109 hatten. Ich weiß, dass Sie schon viel Zeit in Ihre Experimente investiert haben und es ist enttäuschend, dass Sie bisher noch kein positives Ergebnis haben. Ich kann Sie darin bestätigen, dass die Pankreasschnitte auf jeden Fall positive Ergebnisse zeigen sollten. Laut Literatur eignet sich Lungengewebe auch als gute Positivkontrolle; Sie haben sich daher auf jeden Fall die richtigen Kontrollen ausgesucht. Bei der Durchsicht Ihres Protokolls ist mir aufgefallen, dass Sie als Sekundärantikörper ab98696 verwenden: Dies ist ein Antikörper gegen Maus-IgG2a, der noch weiteren Aufreinigungsschritten unterzogen wurde (pre-adsorbed), um eventuell vorhandene Kreuzreaktivitäten zu minimieren. Daher ist es sehr unwahrscheinlich, dass ab98696 den anti-VEGF- Antikörper ab3109 bindet, da dieser Primärantikörper vom IgG1-Isotyp (mit der kappa light chain) ist. Ich möchte Ihnen daher empfehlen, einen anderen Sekundärantikörper zu verwenden (zum Beispiel ab98691 Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html).). Bei einer Inkubation ueber Nacht würden wir zwar eine Inkubationstemperatur von 4ºC empfehlen, da dies zu einem spezifischeren Signal führt. Abgesehen davon sieht Ihr Protokoll aber sehr gut aus und sollte positive Ergebnisse mit ab3109 und Ihren Proben bringen. Bitte lassen Sie mich wissen, ob ich Ihnen helfen konnte und zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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