Goat Anti-Mouse IgG1 heavy chain (FITC) preadsorbed (ab98692)

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- Have you used correct filters? The ab150079 absorption maxima is 650 nm while for FITC (ab98692) it is 495.
- Were the cells permeabilized?
- Is the sequence of insert protein is interpreted. It may well be that the insert sequence is missing which ab128251 binds.
The antibodies ab128251 and a84815 are raised against different part of the immunogen.
- Have you tried optimizing the results by incubating the antibody overnight or by increasing the concentration?

In order for checking the quality of ab150079 you may put a small drop of ab on slide and then seeing it under microscope. If it emits red colour then conjugate is fine and the problem may be with other steps. If it's not then problem is with secondary antibody.

It will be very helpful if you could provide the protcol followed step by step.

Please note that flow cytometry is not a tested application for ab128251 so the Abreview guarantee will not be valid. However if this antibody fails in ELISA, WB and ICC/IF then guarantee will apply as normal product.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

The concentration of this lot is GR91861-1 is 0.85mg/ml. An appropriate isotype control would be ab91353, Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. This product is shipped with a concentration of 1mg/ml. https://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html We recommend use of a pre-absorbed secondary antibody in Flow Cytometry. We have a number of pre-absorbed anti-Mouse IgG1 antibodies which are conjugated to fluorescent molecules for use in Flow. Choice of the appropriate marker will depend upon your experimental set up and flow cytometer. I have complied a short list for you below. Secondary Antibodies: AB98692 (FITC conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-pre-adsorbed-ab98692.html AB136127 (Cy5 conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-gamma-chain-Cy5--pre-adsorbed-ab136127.html AB130785 (AP/Cy7 conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-AllophycocyaninCy7-pre-adsorbed-ab130785.html AB130786 (AP conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-Allophycocyanin-pre-adsorbed-ab130786.html

Thank you for contacting us and your interest in our products.

Both the goat polyclonal anti-mouse IgG (ab97239 and ab98692) should be compatible with the mouse anti-CACNA1H antibody (ab84815) for the experiment you hope to perform. The antibody ab98692 is provided pre-adsorbed. This can be very useful when using multi-color imaging and IHC experiments when several primary antibodies and their corresponding secondary antibodies are used simultaneously. It also reduces the risk of cross-reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. It can therefore help to reduce non-specific staining.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Thank you for your enquiry and your interest in our products.
I have conducted a search for you and found several antibodies against Sarcomeric Alpha Actinin. One of them is ab9465 which has been tested in ICC/IF and it recognizes cardiac muscle in different species (Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Dog, Pig, Xenopus laevis, Fish, Lizard, Snake, Xenopus tropicalis ). We have excellent feedback (3 Abreviews) from our customers who have used it and also specific publications (11). As you can see on the databases, it is a mouse monoclonal antibody and the isotype is IgG1.
For selecting the correct compatible secondary antibodies, I would advise you to use our advanced search engine: https://www.abcam.com/index.html?pageconfig=productmap&cl=918
You can type the target and the host species, isotype, format, specificity, conjugate etc. Have you considered the fluorochromes you wish to use?
Here are a few suggestions:
ab97239: Goat anti-Mouse IgG1 heavy chain (FITC) secondary antibody,
ab98692: Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (FITC), pre-adsorbed
ab72553: Goat polyclonal Secondary Antibody to Mouse IgG1 (SureLight® Allophycocyanin)
Regarding ICC protocol, I would advise you to take a look at the Protocol section at our website. We have a great numbers of general protocols which may be useful for you:
https://www.abcam.com/index.html?pageconfig=popular_protocols
https://www.abcam.com/index.html?pageconfig=resource&rid=11417
https://www.abcam.com/index.html?pageconfig=resource&rid=12129
I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiry and interest.

You could certainly use our advanced search engine specifically designed for secondary antibodies to find the most suitable products for your studies:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918



Since the isotype of this primary antibody is mouse IgG1, so you could select the isotype, target species, host species, format, specificity, conjugate using the drop-down menu.

We have different conjugates such as FITC, DyLight488, Chromeo 488, and here are a few suggestions:

FITC:

ab98692

ab97239

ab11588

You can find some further information about the the generation of our fluorochromes at this site:https://www.abcam.com/index.html?pageconfig=resource&rid=12948

DyLight488:

Affinity purified: ab96875, ab97015, ab96871

Pre-adsorbed: ab96879

Chromeo 488:

ab60313



If you need any further assistance in the future, please do not hesitate to contact me.