Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP) (ab102279)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, ICC, IHC-P
Overview
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Product name
Goat F(ab')2 Anti-Rabbit IgG (Fab')2 (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: WB, ELISA, ICC, IHC-Pmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000).
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ELISA |
1/10000 - 1/100000. (Primary).
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ICC |
1/200 - 1/5000.
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IHC-P |
1/200 - 1/5000.
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Notes |
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WB
1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000). |
ELISA
1/10000 - 1/100000. (Primary). |
ICC
1/200 - 1/5000. |
IHC-P
1/200 - 1/5000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab102279 has been referenced in 3 publications.
- Perez-Valle A et al. Upregulated phospholipase D2 expression and activity is related to the metastatic properties of melanoma. Oncol Lett 23:140 (2022). PubMed: 35340556
- Wang S et al. Interleukin-4 affects the mature phenotype and function of rat bone marrow-derived dendritic cells. Mol Med Rep 12:233-7 (2015). PubMed: 25683957
- Severino P et al. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. BMC Cancer 13:533 (2013). WB . PubMed: 24209638