Rabbit Anti-Human IgA H&L (HRP) (ab8510)
Key features and details
- Rabbit Anti-Human IgA H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: Dot blot, ELISA
Overview
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Product name
Rabbit Anti-Human IgA H&L (HRP)
See all IgA secondary antibodies -
Host species
Rabbit -
Target species
Human -
Specificity
This product does have a minor cross-reactivity to mouse samples.
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Tested applications
Suitable for: Dot blot, ELISAmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/100.
|
|
ELISA |
1/1000.
|
Notes |
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Dot blot
1/100. |
ELISA
1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab8510 has been referenced in 3 publications.
- Lutomski CA et al. Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies. JACS Au 1:1147-1157 (2021). PubMed: 34462738
- Kempsell KE et al. Whole genome protein microarrays for serum profiling of immunodominant antigens of Bacillus anthracis. Front Microbiol 6:747 (2015). ELISA . PubMed: 26322022
- Csorba K et al. Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases. Orphanet J Rare Dis 6:31 (2011). ELISA . PubMed: 21619684