Goat Anti-Mouse IgG1 (HRP) (ab97240)
Key features and details
- Goat Anti-Mouse IgG1 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
Overview
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Product name
Goat Anti-Mouse IgG1 (HRP)
See all IgG1 secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species. -
Tested applications
Suitable for: ICC, ELISA, IHC-P, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab97240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/200 - 1/2000. | |
| ELISA | 1/10000 - 1/100000. | |
| IHC-P | 1/200 - 1/2000. | |
| WB | 1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) (ab97240)
IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat Anti-Mouse IgG1 (HRP) (ab97240)Image from Arnò B et al., PLOS One, Fig. 2.; doi: 10.1371/journal.pone.0068404 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/All lanes : Anti-Topoisomerase I antibody (ab58313)
Lane 1 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 1 minute.
Lane 2 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 2 minutes.
Lane 3 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 4 minutes.
Lane 4 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 8 minutes.
Lane 5 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 1 minute.
Lane 6 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 2 minutes.
Lane 7 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 4 minutes.
Lane 8 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 8 minutes.
Secondary
All lanes : Goat Anti-Mouse IgG1 (HRP) (ab97240)The band corresponding to the enzyme–substrate cleaved complex, has been indicated by an asterisk.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (49)
ab97240 has been referenced in 49 publications.
- Vanderleyden I et al. Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5. Cell Rep 30:611-619.e4 (2020). PubMed: 31968240
- Biram A et al. B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches. Cell Rep 30:1910-1922.e5 (2020). PubMed: 32049020
- Stebegg M et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. Elife 9:N/A (2020). PubMed: 32204792
- Broecker F et al. A mosaic hemagglutinin-based influenza virus vaccine candidate protects mice from challenge with divergent H3N2 strains. NPJ Vaccines 4:31 (2019). PubMed: 31341648
- Xue RY et al. Synthetic Lipopeptide Enhances Protective Immunity Against Helicobacter pylori Infection. Front Immunol 10:1372 (2019). PubMed: 31258538
