Goat Anti-Mouse IgG1 (HRP) (ab97240)

Reviews (1)Q&A (3)References (43)

Overview

  • Product name

    Goat Anti-Mouse IgG1 (HRP)
    See all IgG1 secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species.

  • Tested applications

    Suitable for: ICC, ELISA, IHC-P, WBmore details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200 - 1/2000.
ELISA 1/10000 - 1/100000.
IHC-P 1/200 - 1/2000.
WB 1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) (ab97240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) (ab97240)

    IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Mouse IgG1 (HRP) (ab97240)
    Western blot - Goat Anti-Mouse IgG1 (HRP) (ab97240)Image from Arnò B et al., PLOS One, Fig. 2.; doi: 10.1371/journal.pone.0068404 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
    All lanes : Anti-Topoisomerase I antibody (ab58313)

    Lane 1 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 1 minute.
    Lane 2 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 2 minutes.
    Lane 3 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 4 minutes.
    Lane 4 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 8 minutes.
    Lane 5 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 1 minute.
    Lane 6 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 2 minutes.
    Lane 7 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 4 minutes.
    Lane 8 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 8 minutes.

    Secondary
    All lanes : Goat Anti-Mouse IgG1 (HRP) (ab97240)


    The band corresponding to the enzyme–substrate cleaved complex, has been indicated by an asterisk.

References

This product has been referenced in:

  • Li TZ  et al. Protease-activated receptor-2 regulates glial scar formation via JNK signaling. Physiol Res N/A:N/A (2019). Read more (PubMed: 30628825) »
  • Wang Z  et al. The human DNA topoisomerase I mutant Gly717Asp: Higher religation rate is not always associated with camptothecin resistance. Arch Biochem Biophys 663:165-172 (2019). Read more (PubMed: 30653963) »
See all 43 Publications for this product

Publishing research using ab97240? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Super specific 2nd antibody against mouse IgG1 Heavy Chain only

 Excellent
Abreviews
abreview image
Application
Western blot
This antibody is super specific! It does not react with mouse IgG2a or human IgG, or any IgG light chain I tested so far.

I use it because I work with mouse tumor lysate in western blot. All tumor lysate contains blood, so IgG is always in the lysates. When my protein of interest is at the same molecular weight of IgG light chain, I use this antibody.

The purpose of western blot I show here is to verify if this antibody reacts with light chain of mouse IgG1 specifically, so there is no primary antibody used.

The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.

Abcam user community

Verified customer

Submitted Oct 14 2015

Question

I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1-HRP #ab99774
mouse mAb anti human IgG2-HRP #ab99784
mouse mAb anti human IgG3-HRP #ab99829
mouse mAb anti human IgG4-HRP #ab99817

2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

non-pre-adsorbedvs preadsorbed
anti IgM ab97230vs ab98679
anti IgG1 ab98693vsab97240
anti IgG2a ab97245 vs ab98698
anti IgG2b ab97250 vs ab98703
anti IgG2c ab97255 vs ab98722
anti IgG3 ab97260vsab98708


3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

Many thanks,

Tapan Bhattacharyya
London School of Hygiene & Tropical Medicine

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Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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Question

Hi,
I have a question about antibody from abcam. Does Goat polyclonal
Secondary Antibody to Mouse IgG1 - heavy chain (HRP) cat# ab97240
react with Anti-ERCC1 antibody [3H11] cat# ab43 ?
Thanks

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Answer

Thank you for your enquiry.

I can confirm that ab43ERCC1 antibody [3H11] is a mouse monoclonal,IgG2a isotype.

The ab97240 secondary antibody detects mouse IgG1 isotype, so it would not be suitable for use with ab43. You will need a secondary antibody that either specifically detects mouse IgG2a, or one that detects the more general IgG isotype.

I would be pleased to help find some more suitable secondaries foryou. In order to do so, I would appreciate if you could confirm which application you will be using (for example, WB, IHC-P). I can then find an antibody suitable for your experiments.

I hope this will be helpful and I look forward to hearing from you.

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Question

Thank you very much for your helpful information and time. I am looking for anti-mouse IgG2a and IgG1 secondary antibodies (either HRP- or AP- conjugated) to use for Isotyping of specific mouse serum IgGs against my desired antigen. I searched through your products but I could not find any product. I should say that the secondary antibodies I need must not cross-react with other mouse IgG subtypes. By the way, if you have any mouse IgG isotyping ELISA kit, I really appreciate it if you let me know. I am looking forward to hearing from you. Best Wishes,

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Answer

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/index.html?datasheet=99603 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97237 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/index.html?datasheet=997240 Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/index.html?datasheet=11571 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97245 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97242 Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

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