Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)

Reviews (1)Q&A (17)References (8)

Key features and details

  • Goat Anti-Mouse IgG1 (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Mouse IgG1 (HRP) preadsorbed
    See all IgG1 secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1.

    Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins.

    Less than 1% cross reactivity to human and rat IgG1 was detected. This antibody may cross react with IgG1 from other species.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG1 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)

    IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab98693, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (8)

Publishing research using ab98693? Please let us know so that we can cite the reference in this datasheet.

ab98693 has been referenced in 8 publications.

  • Blokhina EA  et al. Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin. Plants (Basel) 9:N/A (2020). PubMed: 32013187
  • Ozek C  et al. Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice. Sci Rep 8:17293 (2018). PubMed: 30470794
  • Lynn MA  et al. Early-Life Antibiotic-Driven Dysbiosis Leads to Dysregulated Vaccine Immune Responses in Mice. Cell Host Microbe 23:653-660.e5 (2018). PubMed: 29746836
  • Gulati S  et al. Attenuation of opioid tolerance by ETB receptor agonist, IRL-1620, is independent of an accompanied decrease in nerve growth factor in mice. Heliyon 3:e00317 (2017). WB ; Mouse . PubMed: 28626808
  • Liu SA  et al. Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice. J Virol 91:N/A (2017). ELISA . PubMed: 28404844
  • Koirala P  et al. HHLA2, a member of the B7 family, is expressed in human osteosarcoma and is associated with metastases and worse survival. Sci Rep 6:31154 (2016). IHC . PubMed: 27531281
  • Sochalska M  et al. Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival. Cell Death Differ 23:628-39 (2016). WB . PubMed: 26450454
  • Garrett SM  et al. Agonism of the 5-hydroxytryptamine 1F receptor promotes mitochondrial biogenesis and recovery from acute kidney injury. J Pharmacol Exp Ther 350:257-64 (2014). WB . PubMed: 24849926

Customer reviews and Q&As

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1-10 of 18 Abreviews or Q&A

Western blot using the antibody with cell lysate prepared from S. cerevisiae.

 Excellent
Abreviews
abreview image
Application
Western blot
Myc tagged protein 65kDa probed with Monoclonal Myc antibody and later with Mouse secondary Heavy chain specific HRP. Lane 5 is negative control with no Myc tagged protein. The antibody is excellent and recognized the specific band. I highly recommend this antibody.

Abcam user community

Verified customer

Submitted Feb 28 2014

Question

To whom it may concern

We are evaluating gamma delta and CD4+Tcells in chicken’s section by IHC technique and we purchase your primary antibodies (ab25347 and ab25345) and secondary HRP antibody (ab98693).

After looking your website and datasheet, for suitable titer of them, we find that we must set it our self, because there was no titer in your datasheet.

Have you ever set up the titer of these three antibodies? We wish to have your kind suggestion for choosing the suitable concentration.

Best regards

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Answer

Thank you for contacting us.

The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some optimisation.

We offer a table on our web page under technical FAQs that shows the recommended titer for different applications according to the purification of antibodies.

https://www.abcam.com/index.html?pageconfig=technicalfaqs#8


The secondary HRP antibody ab98693 has a recommendation for a titer between 1/200 - 1/2000 for IHC-P. The recommendation can be find on the product data sheet https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-pre-adsorbed-ab98693.pdf.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

ab70: will test in ELISA
send DS link for ab98693

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Answer

Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-hrp-pre-adsorbed-ab98693.html


Here is the discount code for ab70 and trying it in ELISA, as well as additional information about our Abtrial program:
DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab70 in ELISA. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Question

Our customer related that the liquid was all over the pouch.
Abcam Order # 1119930 -- July 09th 2012

Thanks and best regards

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1150356.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

Hello
Our customer received the product ab98693 (PO 123642) damaged.
Is it possible to send another?
Attached the purchase document (#2)
Thanks and best regards

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Answer

Thank you for contacting us.

Could you provide more details about the problem?

- was the vial broken
- was the liquid all over the pouch
- When the product was ordered. Could you provide Abcam purchase order number? The purchase order number PO 123642 does not retrieve any information in our system. Could you provide the date of ordering?

I look forward to hearing from you soon.

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Question

thanks for the reply, could you please confirm the products ab98963,ab97245 andab97250 can be used asELSIAsecondary antibodies.

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Answer

Thank you for your email.

These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

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Question

Thanks for your kindly reply
about my first question, i want to know after incubation of secondary antibody (ab98693) is there any necessity for substrate? do we need anythings else other than DAB or AEC for enzymatic detection ? if we need, could you tell us the name of substrate?

Best regards

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Answer

Thank you for your email.

The ab98693 is conjugated the HRP enzyme and you will need just the substrate in addition. As a substrate, you can for example use the AEC substrate system ab64252 or the DAB substrate Kit ab64238. The DAB and the AEC are already the substrate.

After addition of the substrate, you can counterstain the slides with HE for example. This will give the nice blue and rose staining of the tissue. This is not necessary, will however help to identify the structure. HE staining is very common and we do also have a kit, it has the catalogue number ab128990 https://www.abcam.com/index.html?datasheet=128990 (or use the following: https://www.abcam.com/index.html?datasheet=128990).

Other than that, you will not need anything. You will need mounting medium and coverslips.]

Here are also some links to our online protocols. Feel free to browse them as well.
https://www.abcam.com/index.html?pageconfig=resource&rid=11384
https://www.abcam.com/index.html?pageconfig=resource&rid=11757

I hope this information is helpful. Please do not hesitate to contact us, if I did not respond to your question as expected or if you have any other question.

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Question

Following this email,
1.could you tell us the name of substrate please ?
2.which one you recommend for IHC frozen (DAB or AEC) ?

Best regards

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Answer

Thank you for your email.

As x is not in the office today, I am answering on his behalf.

I can confirm that you can use AEC or DAB depending on your preference and the counterstain of your slides. Both of these substrates are adapted for immunohistochemistry. DAB is brown in color and AEC is red in color. Both of these substrates can be mounted in aqueous mounting medium such as Mowiol for example. In addition to the aqueous mounting medium, DAB can also be mounted in an organic medium (whereas AEC not, as it will solubilize in an organic mediumand result then in a diffuse staining).

As an example image for AEC, I can recommend you to look at the datasheet of our AEC substrate system ab64252, and for DAB, at the datasheet of the DAB substrate Kit ab64238.

https://www.abcam.com/index.html?datasheet=64252 (or use the following: https://www.abcam.com/index.html?datasheet=64252).

https://www.abcam.com/index.html?datasheet=64238 (or use the following: https://www.abcam.com/index.html?datasheet=64238).

I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.

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Question

WB with dog heart samples
sees lots of background
10-80 ug protein loaded
block: 5% milk, tried 1h to overnight
Ab: 1/1000, 1/200, 1/20,000 from 1h to overnight
2nd: ab988693, works so so
suggestions to find out which Ab is the problem:
1) try different secondary Ab (with 20-50 ug protein, 1/1000 dilution)
2) try no primary control

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Answer

Thank you for contacting us.
I am sorry to hear that either one or both antibodies are not working as expected.

As we discussed over the phone, the following suggestions will help us to determine if either one of the antibodies or both of them are not workingcorrectly:

1) Use adifferent secondary antibody (with 20-50 ug protein, 1/1000 dilution)
2) Try a no primary control to see if the secondary antibody contributes to the background. Ideally, the blot should be blank.

Please let me know what results you obtain and I'd be happy to discuss this further in order to resolve the problem.
I wish you good luck andlook forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

We were thinking on your kind advice an after checking your website guidance we finally come to order following antibodies for our IHC-Fr test:
Ab25345 Ab25346 Ab25347 and Ab98693

Before placing order, we highly appreciate your detailed asnwers on our followng quetions:

1- will we need any substrate for completing the reaction after using above primary and secondary antibodies or your kit will be quote enough and complete?

2- which substrate should we apply to observe the immunoreaction, DAB or AEC ?

3- considering our chosen antibodies, which stains are preferred for counterstain tissue level, hematoxylin, methylgreen or any other ?

We duly thank for your kind support in advance.

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Answer

Thank you for contacting us.

1. Yes, a substrate is needed.

2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
> IHC detectionkits 'EXPOSE': https://www.abcam.com/expose (see the 6 first references in thefirst table called"Mouse and rabbit specific kits")
> Steady DAB/Plus (200ml), https://www.abcam.com/ab103723
> AEC Single/Plus (30ml) https://www.abcam.com/ab103742
> DAB Substrate Kit https://www.abcam.com/ab64238
> AEC Substrate System (Ready to Use) https://www.abcam.com/ab64252

3. For the counterstainingI would recommend hematoxylin. Unfortunatelywedonot have such product inour catalog.

Finally,I would recommend the following protocolsforIHC:https://www.abcam.com/index.html?pageconfig=resource&rid=11757

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 18 Abreviews or Q&A

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