Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)

Reviews (1)Q&A (17)References (11)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)

    Key features and details

    • Goat Anti-Mouse IgG1 (HRP) preadsorbed
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC, IHC-P, ELISA, WB

    Overview

    • Product name

      Goat Anti-Mouse IgG1 (HRP) preadsorbed
      See all IgG1 secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1.

      Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins.

      Less than 1% cross reactivity to human and rat IgG1 was detected. This antibody may cross react with IgG1 from other species.

    • Tested applications

      Suitable for: ICC, IHC-P, ELISA, WBmore details

    • Minimal
      cross-reactivity


      Human, Rat more details

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG1 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab98693 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC
    Use at an assay dependent dilution.
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. Similar dilution to primary antibody.
    WB (1)
    1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent
    Notes
    ICC
    Use at an assay dependent dilution.
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. Similar dilution to primary antibody.
    WB
    1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)

      IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab98693, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

      The inset negative control image is secondary-only at 1/500 dilution.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (11)

    Publishing research using ab98693? Please let us know so that we can cite the reference in this datasheet.

    ab98693 has been referenced in 11 publications.

    • Blokhina EA  et al. Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin. Plants (Basel) 9:N/A (2020). PubMed: 32013187
    • Pitts MG  et al. Autoantibody Responses to Apolipoprotein A-I Are Not Diet- or Sex-Linked in C57BL/6 Mice. Immunohorizons 4:455-463 (2020). PubMed: 32759326
    • Shu Z  et al. Viridicatol Isolated from Deep-Sea PenicilliumGriseofulvum Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation. Mar Drugs 18:N/A (2020). PubMed: 33081290
    • Briyal S  et al. Alterations in endothelin receptors following hemorrhage and resuscitation by centhaquin. Physiol Res 67:S199-S214 (2018). PubMed: 29947540
    • Ozek C  et al. Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice. Sci Rep 8:17293 (2018). PubMed: 30470794
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 18 Abreviews or Q&A

    Western blot using the antibody with cell lysate prepared from S. cerevisiae.

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Myc tagged protein 65kDa probed with Monoclonal Myc antibody and later with Mouse secondary Heavy chain specific HRP. Lane 5 is negative control with no Myc tagged protein. The antibody is excellent and recognized the specific band. I highly recommend this antibody.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 28 2014

    Question

    To whom it may concern

    We are evaluating gamma delta and CD4+Tcells in chicken’s section by IHC technique and we purchase your primary antibodies (ab25347 and ab25345) and secondary HRP antibody (ab98693).

    After looking your website and datasheet, for suitable titer of them, we find that we must set it our self, because there was no titer in your datasheet.

    Have you ever set up the titer of these three antibodies? We wish to have your kind suggestion for choosing the suitable concentration.

    Best regards

    Read More
    Answer

    Thank you for contacting us.

    The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some optimisation.

    We offer a table on our web page under technical FAQs that shows the recommended titer for different applications according to the purification of antibodies.

    https://www.abcam.com/index.html?pageconfig=technicalfaqs#8


    The secondary HRP antibody ab98693 has a recommendation for a titer between 1/200 - 1/2000 for IHC-P. The recommendation can be find on the product data sheet https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-pre-adsorbed-ab98693.pdf.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    ab70: will test in ELISA
    send DS link for ab98693

    Read More
    Answer

    Thank you for your phone call.

    The link to the datasheet of the secondary antibody is :

    https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-hrp-pre-adsorbed-ab98693.html


    Here is the discount code for ab70 and trying it in ELISA, as well as additional information about our Abtrial program:
    DISCOUNT CODE: xxx
    Expiration date: xxx
    Value: xxx

    I am very pleased to hear you would like to accept our offer and test ab70 in ELISA. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

    Read More

    Question

    Our customer related that the liquid was all over the pouch.
    Abcam Order # 1119930 -- July 09th 2012

    Thanks and best regards

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1150356.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Hello
    Our customer received the product ab98693 (PO 123642) damaged.
    Is it possible to send another?
    Attached the purchase document (#2)
    Thanks and best regards

    Read More
    Answer

    Thank you for contacting us.

    Could you provide more details about the problem?

    - was the vial broken
    - was the liquid all over the pouch
    - When the product was ordered. Could you provide Abcam purchase order number? The purchase order number PO 123642 does not retrieve any information in our system. Could you provide the date of ordering?

    I look forward to hearing from you soon.

    Read More

    Question

    thanks for the reply, could you please confirm the products ab98963,ab97245 andab97250 can be used asELSIAsecondary antibodies.

    Read More
    Answer

    Thank you for your email.

    These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

    Just let me know if you have further questions.

    Read More

    Question

    Thanks for your kindly reply
    about my first question, i want to know after incubation of secondary antibody (ab98693) is there any necessity for substrate? do we need anythings else other than DAB or AEC for enzymatic detection ? if we need, could you tell us the name of substrate?

    Best regards

    Read More
    Answer

    Thank you for your email.

    The ab98693 is conjugated the HRP enzyme and you will need just the substrate in addition. As a substrate, you can for example use the AEC substrate system ab64252 or the DAB substrate Kit ab64238. The DAB and the AEC are already the substrate.

    After addition of the substrate, you can counterstain the slides with HE for example. This will give the nice blue and rose staining of the tissue. This is not necessary, will however help to identify the structure. HE staining is very common and we do also have a kit, it has the catalogue number ab128990 https://www.abcam.com/Mayers-Hematoxylin-ab128990.html (or use the following: https://www.abcam.com/Mayers-Hematoxylin-ab128990.html).

    Other than that, you will not need anything. You will need mounting medium and coverslips.]

    Here are also some links to our online protocols. Feel free to browse them as well.
    https://www.abcam.com/index.html?pageconfig=resource&rid=11384
    https://www.abcam.com/index.html?pageconfig=resource&rid=11757

    I hope this information is helpful. Please do not hesitate to contact us, if I did not respond to your question as expected or if you have any other question.

    Read More

    Question

    Following this email,
    1.could you tell us the name of substrate please ?
    2.which one you recommend for IHC frozen (DAB or AEC) ?

    Best regards

    Read More
    Answer

    Thank you for your email.

    As x is not in the office today, I am answering on his behalf.

    I can confirm that you can use AEC or DAB depending on your preference and the counterstain of your slides. Both of these substrates are adapted for immunohistochemistry. DAB is brown in color and AEC is red in color. Both of these substrates can be mounted in aqueous mounting medium such as Mowiol for example. In addition to the aqueous mounting medium, DAB can also be mounted in an organic medium (whereas AEC not, as it will solubilize in an organic mediumand result then in a diffuse staining).

    As an example image for AEC, I can recommend you to look at the datasheet of our AEC substrate system ab64252, and for DAB, at the datasheet of the DAB substrate Kit ab64238.

    https://www.abcam.com/AEC-Substrate-Kit-ab64252.html (or use the following: https://www.abcam.com/AEC-Substrate-Kit-ab64252.html).

    https://www.abcam.com/DAB-Substrate-Kit-ab64238.html (or use the following: https://www.abcam.com/DAB-Substrate-Kit-ab64238.html).

    I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.

    Read More

    Question

    WB with dog heart samples
    sees lots of background
    10-80 ug protein loaded
    block: 5% milk, tried 1h to overnight
    Ab: 1/1000, 1/200, 1/20,000 from 1h to overnight
    2nd: ab988693, works so so
    suggestions to find out which Ab is the problem:
    1) try different secondary Ab (with 20-50 ug protein, 1/1000 dilution)
    2) try no primary control

    Read More
    Answer

    Thank you for contacting us.
    I am sorry to hear that either one or both antibodies are not working as expected.

    As we discussed over the phone, the following suggestions will help us to determine if either one of the antibodies or both of them are not workingcorrectly:

    1) Use adifferent secondary antibody (with 20-50 ug protein, 1/1000 dilution)
    2) Try a no primary control to see if the secondary antibody contributes to the background. Ideally, the blot should be blank.

    Please let me know what results you obtain and I'd be happy to discuss this further in order to resolve the problem.
    I wish you good luck andlook forward to hear back from you.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    We were thinking on your kind advice an after checking your website guidance we finally come to order following antibodies for our IHC-Fr test:
    Ab25345 Ab25346 Ab25347 and Ab98693

    Before placing order, we highly appreciate your detailed asnwers on our followng quetions:

    1- will we need any substrate for completing the reaction after using above primary and secondary antibodies or your kit will be quote enough and complete?

    2- which substrate should we apply to observe the immunoreaction, DAB or AEC ?

    3- considering our chosen antibodies, which stains are preferred for counterstain tissue level, hematoxylin, methylgreen or any other ?

    We duly thank for your kind support in advance.

    Read More
    Answer

    Thank you for contacting us.

    1. Yes, a substrate is needed.

    2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
    > IHC detectionkits 'EXPOSE': https://www.abcam.com/expose (see the 6 first references in thefirst table called"Mouse and rabbit specific kits")
    > Steady DAB/Plus (200ml), https://www.abcam.com/ab103723
    > AEC Single/Plus (30ml) https://www.abcam.com/ab103742
    > DAB Substrate Kit https://www.abcam.com/ab64238
    > AEC Substrate System (Ready to Use) https://www.abcam.com/ab64252

    3. For the counterstainingI would recommend hematoxylin. Unfortunatelywedonot have such product inour catalog.

    Finally,I would recommend the following protocolsforIHC:https://www.abcam.com/index.html?pageconfig=resource&rid=11757

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    1-10 of 18 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com