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    goat-mouse-igg2a-heavy-chain-hrp-ab97245.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (40)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

    Key features and details

    • Goat Anti-Mouse IgG2a heavy chain (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-P, ELISA, WB, ICC

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Goat Anti-Mouse IgG2a heavy chain (HRP)
      See all IgG2a secondary antibodies
    • Host species

      Goat
    • Target species

      Mouse
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%.
    • Tested applications

      Suitable for: IHC-P, ELISA, WB, ICCmore details
    • Conjugation

      HRP

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
    • Conjugation notes

      Molar enzyme/ antibody protein ratio is 4:1
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Immunology
      • Immune System Diseases
      • Autoimmune
      • Secondary antibodies
      • anti-Mouse
      • IgG2a
      • Enzyme
      • HRP

    Associated products

    • Substrate reagent

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab97245 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. Similar dilution to primary antibody.
    WB (1)
    1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
    ICC
    Use at an assay dependent dilution.
    Notes
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. Similar dilution to primary antibody.
    WB
    1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
    ICC
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

      IHC image of SSB staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab75927, 0.5µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97245, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

      The inset negative control image is taken from an identical assay without primary antibody.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (40)

    Publishing research using ab97245? Please let us know so that we can cite the reference in this datasheet.

    ab97245 has been referenced in 40 publications.

    • Li Z  et al. Intranasal immunization with a rNMB0315 and combination adjuvants induces protective immunity against Neisseria meningitidis serogroup B in mice. Int Immunopharmacol 93:107411 (2021). PubMed: 33548582
    • Powell AE  et al. A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice. ACS Cent Sci 7:183-199 (2021). PubMed: 33527087
    • Lainšcek D  et al. A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response. Vaccines (Basel) 9:N/A (2021). PubMed: 33925446
    • Chen Y  et al. Case Report: A Novel Heterozygous ZP3 Deletion Associated With Empty Follicle Syndrome and Abnormal Follicular Development. Front Genet 12:690070 (2021). PubMed: 34093671
    • Xu K  et al. Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat spike protein RBD protects mice against COVID-19. Emerg Microbes Infect 10:1574-1588 (2021). PubMed: 34289779
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    React with mouse IgG2a heavy chain strongly

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    I tested with 3 mouse tumor lysates and 3 different IgGs.

    Tumor lysates have been also pre-cleared with protein A/G beads to get rid of most (but not all) IgG.

    No primary antibody was used. This secondary antibody was incubated 1:5000 at room temperature for 1 hour. Here are the results:

    1. Ab97245 reacts with mouse IgG2a heavy chain strongly
    2. Ab97245 reacts with mouse IgG2a light chain slightly, which is the reason I did not give it a 5-star.
    3. Ab97245 does not react with mouse IgG1 or human IgG, neither heavy chain or light chain.

    Abcam user community

    Verified customer

    Submitted Oct 02 2015

    Question

    thanks for the reply, could you please confirm the products ab98963,ab97245 andab97250 can be used asELSIAsecondary antibodies.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for your email.

    These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

    Just let me know if you have further questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    I have some specifictechnical questions regarding your secondary antibodies:

    1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

    mouse mAb anti human IgG1-HRP #ab99774
    mouse mAb anti human IgG2-HRP #ab99784
    mouse mAb anti human IgG3-HRP #ab99829
    mouse mAb anti human IgG4-HRP #ab99817

    2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

    non-pre-adsorbedvs preadsorbed
    anti IgM ab97230vs ab98679
    anti IgG1 ab98693vsab97240
    anti IgG2a ab97245 vs ab98698
    anti IgG2b ab97250 vs ab98703
    anti IgG2c ab97255 vs ab98722
    anti IgG3 ab97260vsab98708


    3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

    Many thanks,

    Tapan Bhattacharyya
    London School of Hygiene & Tropical Medicine

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

    For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

    anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

    anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

    anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

    anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

    anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

    anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

    Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Question

    Thank you very much for your helpful information and time. I am looking for anti-mouse IgG2a and IgG1 secondary antibodies (either HRP- or AP- conjugated) to use for Isotyping of specific mouse serum IgGs against my desired antigen. I searched through your products but I could not find any product. I should say that the secondary antibodies I need must not cross-react with other mouse IgG subtypes. By the way, if you have any mouse IgG isotyping ELISA kit, I really appreciate it if you let me know. I am looking forward to hearing from you. Best Wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2011

    Answer

    Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-HRP-preadsorbed-ab99603.html Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Alkaline-Phosphatase-ab97237.html Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Alkaline-Phosphatase-ab97237.html Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/Rat-monoclonal-Mouse-IgG2a-heavy-chain-HRP-LO-MG2a-9-ab11571.html Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-HRP-ab97245.html Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-Alkaline-Phosphatase-ab97242.html Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

    Read More

    Abcam Scientific Support

    Answered on Sep 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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