Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

Abreviews (1)Q&A (3)Specific References (14)

Overview

  • Product name
    Goat Anti-Mouse IgG2a heavy chain (HRP)
    See all IgG2a secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%.
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
ICC Use at an assay dependent dilution.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

    IHC image of SSB staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab75927, 0.5µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97245, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Hasham MG  et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). ELISA . Read more (PubMed: 28250051) »
  • Carvajal-Hausdorf DE  et al. Objective measurement and clinical significance of IDO1 protein in hormone receptor-positive breast cancer. J Immunother Cancer 5:81 (2017). Read more (PubMed: 29037255) »

See all 14 Publications for this product

Publishing research using ab97245? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
Votes: Highest First

React with mouse IgG2a heavy chain strongly

 Good
Abreviews
Application
Western blot
I tested with 3 mouse tumor lysates and 3 different IgGs.

Tumor lysates have been also pre-cleared with protein A/G beads to get rid of most (but not all) IgG.

No primary antibody was used. This secondary antibody was incubated 1:5000 at room temperature for 1 hour. Here are the results:

1. Ab97245 reacts with mouse IgG2a heavy chain strongly
2. Ab97245 reacts with mouse IgG2a light chain slightly, which is the reason I did not give it a 5-star.
3. Ab97245 does not react with mouse IgG1 or human IgG, neither heavy chain or light chain.
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Verified customer

Submitted Oct 02 2015

thanks for the reply, could you please confirm the products ab98963,ab97245 andab97250 can be used asELSIAsecondary antibodies.

Thank you for your email.

These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1...

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Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Thank you very much for your helpful information and time. I am looking for anti-mouse IgG2a and IgG1 secondary antibodies (either HRP- or AP- conjugated) to use for Isotyping of specific mouse serum IgGs against my desired antigen. I searched through ...

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Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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