Overview

  • Product name

    Goat Anti-Mouse IgG2a heavy chain (HRP)
    See all IgG2a secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%.
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
ICC Use at an assay dependent dilution.

Images

  • IHC image of SSB staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab75927, 0.5µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97245, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:

  • Liu C  et al. The mucosal adjuvant effect of plant polysaccharides for induction of protective immunity against Helicobacter pylori infection. Vaccine 37:1053-1061 (2019). Read more (PubMed: 30665774) »
  • Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). Read more (PubMed: 30787986) »
See all 19 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
I tested with 3 mouse tumor lysates and 3 different IgGs.

Tumor lysates have been also pre-cleared with protein A/G beads to get rid of most (but not all) IgG.

No primary antibody was used. This secondary antibody was incubated 1:5000 at room temperature for 1 hour. Here are the results:

1. Ab97245 reacts with mouse IgG2a heavy chain strongly
2. Ab97245 reacts with mouse IgG2a light chain slightly, which is the reason I did not give it a 5-star.
3. Ab97245 does not react with mouse IgG1 or human IgG, neither heavy chain or light chain.

Abcam user community

Verified customer

Submitted Oct 02 2015

Answer

Thank you for your email.

These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

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Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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Answer

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. Please have a look at them and do not hesitate to contact me again should you need any more information. The Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (HRP), pre-adsorbed (ab99603) has been tested by ELISA against a panel of purified Mouse Immunoglobulins including IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA. This product is positive for IgG1 and has minimal reactivity with the other Mouse Immunoglobulins. https://www.abcam.com/index.html?datasheet=99603 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (AP) (ab97237) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97237 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP) (ab97240) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species https://www.abcam.com/index.html?datasheet=997240 Rat monoclonal [LO-MG2a-9] Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab11571) ab11571 recognises murine gamma 2a immunoglobulin heavy chain, and does not cross-react with other murine immunoglobulin classes or subclasses. https://www.abcam.com/index.html?datasheet=11571 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP) (ab97245) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97245 Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (AP) (ab97242) By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%. https://www.abcam.com/index.html?datasheet=97242 Unfortunately we do not have an immunoglobulin isotyping ELISA kit for the moment in our catalog. I have passed this feedback on and I hope we will have one in the future. I hope this information is helpful. Please do not hesitate to contact me again should you have any other question. I wish you a great weekend!

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