Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed (ab98698)

Overview

  • Product name
    Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed
    See all IgG2a secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (HRP), pre-adsorbed
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2a was detected. This antibody may cross react with IgG2a from other species.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Human, Rat more details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.05% Proclin
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2a was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Primary
WB 1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent

References

This product has been referenced in:
  • Norton TS  et al. The Aspergillus fumigatus farnesyltransferase ß-subunit, RamA, mediates growth, virulence, and antifungal susceptibility. Virulence 8:1401-1416 (2017). WB . Read more (PubMed: 28489963) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.

The antibody we discussed, ab98698, is an appropriate antibody for detection of the anti-CAPZB antibody, ab122980.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98698). <...

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Following on my previous message about the secondary antibodies you are interested in, the anit-human IgG isotypes are tested on a Human IgG subclass plate (IgG1, IgG2, IgG3, and IgG4) to ensure activity and minimal cross reactivity with the other IgG ...

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Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Thank you for your inquiry. In case the customer would like to perform a sandwich ELISA (sELISA) for the EBV viral capsid antigen, I would suggest to test the antibody clones [2E08] and [8.F.90] (ab48414 and ab32803, see below). To our knowledge, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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