Goat Anti-Mouse IgG2a heavy chain (PE/Cy7®) preadsorbed (ab130787)
Key features and details
- Goat Anti-Mouse IgG2a heavy chain (PE/Cy7®) preadsorbed
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA, WB
Overview
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Product name
Goat Anti-Mouse IgG2a heavy chain (PE/Cy7®) preadsorbed
See all IgG2a secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
ab130787 reacts with the heavy chain of Mouse IgG2a as demonstrated by ELISA, FLISA, and/or Flow Cytometry. Goat Anti-Mouse IgG2a heavy chain (PE/Cy7 ®) preadsorbed shows minimal cross reactivity with Human immunoglobulins.
Cross absorption: Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled Human sera and purified Human paraproteins. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISA, WBmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Mouse IgG2a paraproteins
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Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.1
Preservative: 0.1% Sodium azide
Constituents: PBS, Sucrose -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab130787 is purified by affinity chromatography on Mouse IgG2a covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab130787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | Use at an assay dependent concentration. | |
ICC/IF | Use 0.1µg for 106 cells. | |
ELISA | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab130787 has been referenced in 2 publications.
- Ricklin ME et al. Virus replicon particle vaccines expressing nucleoprotein of influenza A virus mediate enhanced inflammatory responses in pigs. Sci Rep 7:16379 (2017). PubMed: 29180817
- Kaibuchi N et al. Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation. Regen Ther 6:83-89 (2017). PubMed: 30271842