Goat Anti-Mouse IgG2a heavy chain (PE/Cy7 ®) preadsorbed (ab130787)

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Overview

  • Product name

    Goat Anti-Mouse IgG2a heavy chain (PE/Cy7 ®) preadsorbed
    See all IgG2a secondary antibodies

  • Description

    Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (PE/Cy7 ®), pre-adsorbed

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    ab130787 reacts with the heavy chain of Mouse IgG2a as demonstrated by ELISA, FLISA, and/or Flow Cytometry. Goat Anti-Mouse IgG2a heavy chain (PE/Cy7 ®) preadsorbed shows minimal cross reactivity with Human immunoglobulins.
    Cross absorption: Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled Human sera and purified Human paraproteins.

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Mouse IgG2a paraproteins

  • Conjugation

    PE/Cy7 ®. Ex: 496nm, Em: 774nm

Properties

Applications

Our Abpromise guarantee covers the use of ab130787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use 0.1µg for 106 cells.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

This product has been referenced in:

  • Ricklin ME  et al. Virus replicon particle vaccines expressing nucleoprotein of influenza A virus mediate enhanced inflammatory responses in pigs. Sci Rep 7:16379 (2017). Read more (PubMed: 29180817) »
  • Kaibuchi N  et al. Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation. Regen Ther 6:83-89 (2017). Read more (PubMed: 30271842) »
See all 2 Publications for this product

Publishing research using ab130787? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I need second Ab with fluorochrome specific for primary mouse IgG2a without crossreactivity to human Ig and mouse IgG. Is pruduct 130787 suitable for this purpose? I have human cells bound with two primary mouse mAbs (mouse IgG1 and IgG2a) in human serum. I need detect mouse IgG2a only.

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Answer


In this case, I would recommend using a pre-adsorbed secondary antibody thus reducing the risk of cross-reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. Please so the following link for more information on pre-adsorbed antibodies: https://www.abcam.com/index.html?pageconfig=resource&rid=12956

ab130787 is indeed pre-adsorbed to Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled Human sera and purified Human paraproteins. This therefore means that it should not react with any of immunoglobulins.

This Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (PE/Cy7 ®), pre-adsorbed (ab130787) would therefore be appropriate as would Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (Allophycocyanin/Cy7®), pre-adsorbed (ab130788).

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