Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)

Submit a review Q&A (3)References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)

    Key features and details

    • Goat Anti-Mouse IgG2b heavy chain (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC, IHC-P, ELISA, WB

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    Overview

    • Product name

      Goat Anti-Mouse IgG2b heavy chain (HRP)
      See all IgG2b secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with other immunoglobulins is less than 2%.

    • Tested applications

      Suitable for: ICC, IHC-P, ELISA, WBmore details

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

    • Conjugation notes

      Molar enzyme/ antibody protein ratio is 4:1

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab97250 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC
    Use at an assay dependent dilution.
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. Similar dilution to primary antibody.
    WB
    1/2000 - 1/25000. WB-Colorimetric 1/2000 - 1/20000; WB-Chemiluminescent 1/5000 - 1/25000
    Notes
    ICC
    Use at an assay dependent dilution.
    IHC-P
    1/200 - 1/2000.
    ELISA
    1/10000 - 1/100000. Similar dilution to primary antibody.
    WB
    1/2000 - 1/25000. WB-Colorimetric 1/2000 - 1/20000; WB-Chemiluminescent 1/5000 - 1/25000

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)

      IHC image of Coilin staining in human testis formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab87913, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97250, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

      The inset negative control image is taken from an identical assay without primary antibody.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (15)

    Publishing research using ab97250? Please let us know so that we can cite the reference in this datasheet.

    ab97250 has been referenced in 15 publications.

    • Wang X  et al. TFEB-NF-?B inflammatory signaling axis: a novel therapeutic pathway of Dihydrotanshinone I in doxorubicin-induced cardiotoxicity. J Exp Clin Cancer Res 39:93 (2020). PubMed: 32448281
    • Evtushenko EA  et al. Plant virus particles with various shapes as potential adjuvants. Sci Rep 10:10365 (2020). PubMed: 32587281
    • Gupta A  et al. A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis. PLoS Pathog 16:e1009030 (2020). PubMed: 33175909
    • Phan A  et al. Stromalin Constrains Memory Acquisition by Developmentally Limiting Synaptic Vesicle Pool Size. Neuron 101:103-118.e5 (2019). PubMed: 30503644
    • Nika L  et al. An HER2-Displaying Virus-Like Particle Vaccine Protects from Challenge with Mammary Carcinoma Cells in a Mouse Model. Vaccines (Basel) 7:N/A (2019). PubMed: 31137559
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    thanks for the reply, could you please confirm the products ab98963,ab97245 andab97250 can be used asELSIAsecondary antibodies.

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    Answer

    Thank you for your email.

    These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

    Just let me know if you have further questions.

    Read More

    Question

    using this secondary at 1:10000 produced a totally black western blot; primary has worked fine with a different secondary; sample has worked with other antibodies too

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    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab98703.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

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    Question

    I have some specifictechnical questions regarding your secondary antibodies:

    1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

    mouse mAb anti human IgG1-HRP #ab99774
    mouse mAb anti human IgG2-HRP #ab99784
    mouse mAb anti human IgG3-HRP #ab99829
    mouse mAb anti human IgG4-HRP #ab99817

    2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

    non-pre-adsorbedvs preadsorbed
    anti IgM ab97230vs ab98679
    anti IgG1 ab98693vsab97240
    anti IgG2a ab97245 vs ab98698
    anti IgG2b ab97250 vs ab98703
    anti IgG2c ab97255 vs ab98722
    anti IgG3 ab97260vsab98708


    3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

    Many thanks,

    Tapan Bhattacharyya
    London School of Hygiene & Tropical Medicine

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    Answer

    Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

    For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

    anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

    anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

    anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

    anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

    anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

    anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

    Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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