Goat Anti-Mouse IgG2c heavy chain (Biotin) (ab97253)
Key features and details
- Goat Anti-Mouse IgG2c heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Overview
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Product name
Goat Anti-Mouse IgG2c heavy chain (Biotin)
See all IgG2c secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunogloublin serum proteins. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250 - 1/2500.
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ICC/IF |
1/100 - 1/500.
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ELISA |
1/10000 - 1/200000. (Primary ELISA)
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WB |
1/10000 - 1/200000. (Chemiluminescent)
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Notes |
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IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. (Primary ELISA) |
WB
1/10000 - 1/200000. (Chemiluminescent) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab97253 has been referenced in 3 publications.
- Daniel L et al. Stromal structure remodeling by B lymphocytes limits T cell activation in lymph nodes of Mycobacterium tuberculosis-infected mice. J Clin Invest 132:N/A (2022). PubMed: 36317628
- Counoupas C et al. A single dose, BCG-adjuvanted COVID-19 vaccine provides sterilising immunity against SARS-CoV-2 infection. NPJ Vaccines 6:143 (2021). PubMed: 34848711
- Counoupas C et al. Mucosal delivery of a multistage subunit vaccine promotes development of lung-resident memory T cells and affords interleukin-17-dependent protection against pulmonary tuberculosis. NPJ Vaccines 5:105 (2020). PubMed: 33298977