Goat Anti-Mouse IgG2c heavy chain (HRP) (ab97255)

Submit a review Q&A (1)References (6)

Overview

  • Product name

    Goat Anti-Mouse IgG2c heavy chain (HRP)
    See all IgG2c secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunogloublin serum proteins.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.1% CMIT/MIT 3:1
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary ELISA)
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

References

This product has been referenced in:

  • Yang M  et al. Plant-produced Zika virus envelope protein elicits neutralizing immune responses that correlate with protective immunity against Zika virus in mice. Plant Biotechnol J 16:572-580 (2018). Read more (PubMed: 28710796) »
  • Bian H  et al. Beneficial effects of extracts from Lucilia sericata maggots on burn wounds in rats. Mol Med Rep 16:7213-7220 (2017). Read more (PubMed: 28944889) »
See all 6 Publications for this product

Publishing research using ab97255? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1-HRP #ab99774
mouse mAb anti human IgG2-HRP #ab99784
mouse mAb anti human IgG3-HRP #ab99829
mouse mAb anti human IgG4-HRP #ab99817

2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

non-pre-adsorbedvs preadsorbed
anti IgM ab97230vs ab98679
anti IgG1 ab98693vsab97240
anti IgG2a ab97245 vs ab98698
anti IgG2b ab97250 vs ab98703
anti IgG2c ab97255 vs ab98722
anti IgG3 ab97260vsab98708


3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

Many thanks,

Tapan Bhattacharyya
London School of Hygiene & Tropical Medicine

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Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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