Key features and details
- Goat Anti-Mouse IgG3 heavy chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat Anti-Mouse IgG3 heavy chain (Alkaline Phosphatase)
See all IgG3 secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
Our Abpromise guarantee covers the use of ab97257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/1000 - 1/10000. (Primary).|
|WB||1/1000 - 1/10000. (Chemiluminescent).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97257 has not yet been referenced specifically in any publications.