Key features and details
- Goat Anti-Mouse IgG3 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Product nameGoat Anti-Mouse IgG3 heavy chain (Biotin)
See all IgG3 secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%.
Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Our Abpromise guarantee covers the use of ab97258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/2500.|
|ICC/IF||1/100 - 1/500.|
|ELISA||1/10000 - 1/200000. (Primary).|
|WB||1/10000 - 1/200000. (Chemiluminescent).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97258 has been referenced in 1 publication.
- Prost S et al. Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections. PLoS One 11:e0162419 (2016). ICC/IF . PubMed: 27632367