Goat Anti-Mouse IgG+IgM H&L (18nm Gold) preadsorbed (ab105290)

Overview

  • Product name

    Goat Anti-Mouse IgG+IgM H&L (18nm Gold) preadsorbed
    See all IgG + IgM secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: Electron Microscopy, WB, ELISAmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human more details
  • Conjugation

    Gold 18nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 8.50
    Preservative: 0.05% Sodium azide
    Constituents: 1.5% BSA, 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.381% Sodium borate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy 1/10 - 1/20.
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

References

ab105290 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



We do not determine the antibody concentration for this gold conjugated antibody because during preparation monomers from multimers are separated on density gradients. We do not have any reliable way to measure the antibody concentration on the monomers because the preparation is stabilized with albumin before and after centrifugation.

The colloidal gold-antibody complex is formed by passive adsorption. We express the concentration of the complex in terms of adjusted OD at 520 nm; the complex is tested for antigen binding and the reported OD at 520nm reflects those gold particles to which active antibody is bound. For our gold conjugates, the OD at 520 nm is 2.0. We do not have a value for the molar ratio of gold particles to antibody.

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