Overview

  • Product name

    Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed
    See all IgG + IgM secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    This antibody reacts with Mouse IgG and IgM heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Mouse IgG and Mouse IgM.
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human more details
  • Immunogen

    Full length IgG and IgM molecules (Mouse)

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    10-20 BAC (Biotinamidocaproate N-Hydroxysuccinimide Ester) molecules per IgG molecule
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/10000.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent dilution.

Images

  • Ab47844 was used at dilution 1/1000 with the primary antibody ab24575 in ICC/IF. See the review on ab24575.

References

This product has been referenced in:

  • Zhao J  et al. Effects of paclitaxel intervention on pulmonary vascular remodeling in rats with pulmonary hypertension. Exp Ther Med 17:1163-1170 (2019). Read more (PubMed: 30679989) »
  • Gao P  et al. The therapeutic effects of traditional Chinese medicine Fusu agent in LPS-induced acute lung injury model rats. Drug Des Devel Ther 12:3867-3878 (2018). Read more (PubMed: 30518997) »
See all 2 Publications for this product

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