Key features and details
- Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, ICC/IF, IHC-P
Product nameGoat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed
See all IgG + IgM secondary antibodies
SpecificityThis antibody reacts with Mouse IgG and IgM heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Mouse IgG and Mouse IgM.
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-Pmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length IgG and IgM molecules (Mouse)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes10-20 BAC (Biotinamidocaproate N-Hydroxysuccinimide Ester) molecules per IgG molecule
Our Abpromise guarantee covers the use of ab47844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/100000.|
|WB||1/2000 - 1/10000.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab47844 has been referenced in 2 publications.
- Zhao J et al. Effects of paclitaxel intervention on pulmonary vascular remodeling in rats with pulmonary hypertension. Exp Ther Med 17:1163-1170 (2019). PubMed: 30679989
- Gao P et al. The therapeutic effects of traditional Chinese medicine Fusu agent in LPS-induced acute lung injury model rats. Drug Des Devel Ther 12:3867-3878 (2018). PubMed: 30518997