Overview

  • Product name

    Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed
    See all IgG + IgM secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Mouse IgG and Mouse IgM.
  • Tested applications

    Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Electron Microscopymore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human more details
  • Immunogen

    Full length native proteins (purified) (Mouse)

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    IgG and protease free
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
ELISA 1/5000 - 1/40000.
WB 1/2000 - 1/10000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
Electron Microscopy Use at an assay dependent dilution.

Images

  • Ab47827 was used at dilution 1/500 with the primary antibody ab2869 in IHC-P. See the review on ab2869.

References

This product has been referenced in:

  • Yi L  et al. MicroRNA-130a inhibits growth and metastasis of osteosarcoma cells by directly targeting ZEB1. Mol Med Rep 16:3606-3612 (2017). Read more (PubMed: 28714003) »
  • Wang SK  et al. Fam83h null mice support a neomorphic mechanism for human ADHCAI. Mol Genet Genomic Med 4:46-67 (2016). WB . Read more (PubMed: 26788537) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry.

I am happy to confirm that ab47827 (Goat anti-Mouse IgG + IgM H&L (HRP) secondary antibody) is a suitable secondary antibody for ab3227 and ab22045.

I would like to infirm you that wedo notshipdirectly to Pakistan.

Please contact our trusted distributor: Friends Scientific Corporation (see address below)

I wish you good luck with your research.

Friends Scientific Corporation
3-B Ali Continental,
1-Mozang Road
Lahore
54000
Pakistan
Tel 4237236176 Fax 4237321500 Email mailto:friends1@wol.net.pk

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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