Overview

  • Product name
    Goat Anti-Mouse IgM H&L
    See all IgM secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    The affinity purified anti IgM mu antibodies only react with IgM by immunodiffusion and Immunoelectrophoretic techniques.
  • Tested applications
    Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details
  • Immunogen

    Goats were immunized with highly purified normal mouse IgM in Freund's adjuvant

Properties

Applications

Our Abpromise guarantee covers the use of ab9167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

This product has been referenced in:
  • Fradkin AH  et al. UV photodegradation of murine growth hormone: Chemical analysis and immunogenicity consequences. Eur J Pharm Biopharm N/A:N/A (2014). Read more (PubMed: 24758742) »
  • Rosser EC  et al. Regulatory B cells are induced by gut microbiota-driven interleukin-1ß and interleukin-6 production. Nat Med 20:1334-9 (2014). Read more (PubMed: 25326801) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your e-mail. We are very sorry to hear that you are having problem with this antibody ab9167. We have two new batches (different from 74485) in our stock and will send one vial to you - free of charge. Please do let us know how you are getting on with it. If you say that the new batch works as it should do, then we will send 6 more vials or offer a credit note whichever you would prefer. First, we would really like to find out if the new batch works properly in your experimental system. For your information, we have already placed a new order for you and your new order number is 58591. We look forward to hearing from you soon.

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Question
Answer

Thanks for your patience. Ab9167 - Goat polyclonal to Mouse IgM - is unconjugated. If one of our antibodies is conjugated, it always states that on the datasheet (under conjugation notes) and in the name/description (such as ab5999 - Goat polyclonal to Mouse IgG + IgM + IgA H&L- F(ab)2 Fragment (FITC)). My understanding is that some researchers prefer to conjugate an antibody themselves (for example with some sort of custom conjugate that is not commercially available) and so they would purchase something like this. If you have any more questions, please let me know.

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Answer

Thank you for your enquiry. IgMs can be used as primary antibodies for IF and WB. In the case of ab9167, this antibody has yet to be tested in IF. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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