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  1. Link

    goat-mouse-igm-mu-chain-alexa-fluor-488-ab150121.pdf

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Immunology Immunoglobulins Heavy Chain IgM
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Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (20)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
  • Alexa Fluor® - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)

Key features and details

  • Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgM
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 568 Alexa Fluor® 647 Alkaline Phosphatase APC Biotin DyLight® 488 DyLight® 594 FITC Gold 18nm Gold 6nm HRP Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)
    See all IgM secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgM
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgM
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Secondary antibodies
    • anti-Mouse
    • IgM
    • Fluorophore
    • Alexa Fluor® 488

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
ICC/IF (1)
Use a concentration of 1 - 2 µg/ml.
Flow Cyt (1)
1/2000.
IHC-P (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
1/2000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)

    ICC/IF image of ab20346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20346, 1/500) overnight at +4°C. The secondary antibody (green) was ab150121 Alexa Fluor® 488 goat anti-mouse IgM - mu chain used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
    Alexa Fluor® - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (20)

Publishing research using ab150121? Please let us know so that we can cite the reference in this datasheet.

ab150121 has been referenced in 20 publications.

  • Feng Y  et al. Cell relay-delivery improves targeting and therapeutic efficacy in tumors. Bioact Mater 6:1528-1540 (2021). PubMed: 33294731
  • Dong H  et al. Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering. Tissue Eng Part C Methods 27:253-263 (2021). PubMed: 33798009
  • Dill V  et al. Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire. PLoS One 16:e0246610 (2021). PubMed: 34086711
  • Liu RJ  et al. DnaJA4 is involved in responses to hyperthermia by regulating the expression of F-actin in HaCaT cells. Chin Med J (Engl) 134:456-462 (2020). PubMed: 32925288
  • Anderson-Baron M  et al. Suppression of Hypertrophy During in vitro Chondrogenesis of Cocultures of Human Mesenchymal Stem Cells and Nasal Chondrocytes Correlates With Lack of in vivo Calcification and Vascular Invasion. Front Bioeng Biotechnol 8:572356 (2020). PubMed: 33469528
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Works well (dilution 1:1000)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
I had no problems in using this secondary at the dilution of 1:1000.
The picture shows staining of a mouse derived breast cancer cell line.

Abcam user community

Verified customer

Submitted Sep 11 2017

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