Overview

  • Product name

    Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)
    See all IgM secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488)
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    This antibody is specific to Mouse IgM mu chain
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgM
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Images

  • ICC/IF image of ab20346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20346, 1/500) overnight at +4°C. The secondary antibody (green) was ab150121 Alexa Fluor® 488 goat anti-mouse IgM - mu chain used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

References

This product has been referenced in:

  • Li N  et al. Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells. Stem Cell Res Ther 10:184 (2019). Read more (PubMed: 31234925) »
  • Furusho A  et al. Involvement of B Cells, Immunoglobulins, and Syk in the Pathogenesis of Abdominal Aortic Aneurysm. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29545260) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-2 of 2 Abreviews

Great

Excellent
Abreviews
Application
Flow Cytometry
This antibody worked great for flow against my primary IgM mouse antibody. The signal was strong and clean.

Miss. Kara Shumansky

Verified customer

Submitted May 06 2019

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
I had no problems in using this secondary at the dilution of 1:1000.
The picture shows staining of a mouse derived breast cancer cell line.

Abcam user community

Verified customer

Submitted Sep 11 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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