Goat Anti-Mouse IgM mu chain (APC) preadsorbed (ab130780)
Key features and details
- Goat Anti-Mouse IgM mu chain (APC) preadsorbed
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, WB, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgM mu chain (APC) preadsorbed
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
ab130780 reacts with the heavy chain of mouse IgM. Goat Anti-Mouse IgM mu chain (Allophycocyanin) preadsorbed shows minimal cross reactivity with Human immunoglobulins.
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Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Full length protein corresponding to Mouse IgM. Mouse IgM paraproteins
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Conjugation
APC. Ex: 645nm, Em: 660nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, Sucrose
Sucrose is added as a stabilizing agent. -
Concentration information loading... -
Purification notes
ab130780 is purified by affinity chromatography on mouse IgM covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
ab130780 is preadsorbed against Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled Human sera and purified Human paraproteins. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use 0.1µg for 106 cells.
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| WB |
Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.
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| ELISA |
Use at an assay dependent dilution.
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| Notes |
|---|
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ICC/IF
Use 0.1µg for 106 cells. |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 49 kDa. |
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ELISA
Use at an assay dependent dilution. |
Images
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ELISA - Goat Anti-Mouse IgM mu chain (Allophycocyanin) preadsorbed (ab130780)ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ab130780.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab130780 has not yet been referenced specifically in any publications.