Overview

  • Product name

    Goat Anti-Mouse IgM mu chain (FITC)
    See all IgM secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab97229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids which have carboxy and amine groups in their side-chains, such as Arginine, Lysine, Glutamate or Aspartate. Therefore, the coupling reaction with FITC that usually takes place via free amine groups forming a stable thiourea bond (as for example with our EasyLink kits) is absolutely feasible for the anti-Enrofloxacin/Ciprofloxacin antibody ab21035. However, as the Goat polyclonal Secondary antibody ab97230 is already conjugated to HRP, I would not recommend to label this antibody with FITC. As alternative, I would suggest using an unconjugated antibody such as ab98671: Click here (or use the following: https://www.abcam.com/index.html?datasheet=98671). We also have a Goat polyclonal Secondary antibody to Mouse IgM (mu chain) available that is already FITC-conjugated (ab97229): Click here (or use the following: https://www.abcam.com/index.html?datasheet=97229). I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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