Overview

  • Product name
    Goat Anti-Mouse IgM mu chain (HRP)
    See all IgM secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP)
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: ELISA, WB, ICC, IHC-Pmore details
  • Conjugation
    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab97230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000. (Primary ELISA)
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000)
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.

Images

  • IHC image of Hsp70 staining in human lung formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab5442, 1/2000 dilution overnight at +4°C. An HRP-conjugated secondary (ab97230, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Jiang Y  et al. Aberrant O-glycosylation contributes to tumorigenesis in human colorectal cancer. J Cell Mol Med 22:4875-4885 (2018). Read more (PubMed: 29999571) »
  • Stark AK  et al. PI3Kd hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner. Nat Commun 9:3174 (2018). Read more (PubMed: 30093657) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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Answer

Thank you for contacting us.

I am sorry but I do not fully understand what the customer is hoping to achieve and have thereforefound it difficult to answer your question. How are they testing the antibodies with nanoparticles?

It is unlikely that the antibodies ab97230or ab21035 would bind to simple amine groups but there may be some non-specific background binding depending on the protocol they are using.

If you could explain more fully what the customer is doing and what they are trying to achieve it may be that I can be of more help.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids which have carboxy and amine groups in their side-chains, such as Arginine, Lysine, Glutamate or Aspartate. Therefore, the coupling reaction with FITC that usually takes place via free amine groups forming a stable thiourea bond (as for example with our EasyLink kits) is absolutely feasible for the anti-Enrofloxacin/Ciprofloxacin antibody ab21035. However, as the Goat polyclonal Secondary antibody ab97230 is already conjugated to HRP, I would not recommend to label this antibody with FITC. As alternative, I would suggest using an unconjugated antibody such as ab98671: Click here (or use the following: https://www.abcam.com/index.html?datasheet=98671). We also have a Goat polyclonal Secondary antibody to Mouse IgM (mu chain) available that is already FITC-conjugated (ab97229): Click here (or use the following: https://www.abcam.com/index.html?datasheet=97229). I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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Answer

Thank you for your enquiry. I am sorry the customer has some concerns regarding the results of their experiment. We would not recommend to store the antigen-antibody samples in distilled water. The sample and the antibody binding are likely to be much more stable in PBS.  I would also recommend to store this at 4oC, and not for longer than overnight at 4oC. I am sorry these antibodies have not been tested in this application, so regrettably we are not able to confirm and guarantee how well they would work in this experiment or have a testing protocol.  If the customer has some background, I can recommend to try optimizing the amount of antibody and sample used.  Also to ensure that wash steps are included.  Has the customer tested the antibody in ELISA, which is a tested and guaranteed application? With regards to the binding ration of primary and secondary antibody, I am sorry this will not have been assessed in the laboratory, and will also be different for different primary antibody:secondary antibody combination.   These two antibodies have not specifically been tested in an experiment together as far as we are aware.  Also, the secondary is a polyclonal, so this increases the variability of the number of secondary antibodies bound to each individual primary antibody. I hope this information will be helpful to you. if you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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