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Goat Anti-Mouse IgM mu chain (HRP) preadsorbed (ab98679)

Submit a review Q&A (2)References (1)

Key features and details

  • Goat Anti-Mouse IgM mu chain (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, WB, ELISA, IHC-P

Overview

  • Product name

    Goat Anti-Mouse IgM mu chain (HRP) preadsorbed
    See all IgM secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC, WB, ELISA, IHC-Pmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    HRP

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000. Chemiluminescent: 1/5000 - 1/25000).
ELISA
1/10000 - 1/100000. (as Primary).
IHC-P
1/200 - 1/2000.
Notes
ICC
Use at an assay dependent dilution.
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000. Chemiluminescent: 1/5000 - 1/25000).
ELISA
1/10000 - 1/100000. (as Primary).
IHC-P
1/200 - 1/2000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab98679? Please let us know so that we can cite the reference in this datasheet.

ab98679 has been referenced in 1 publication.

  • Tanaka H  et al. Human colorectal cancer-associated carbohydrate antigen on annexin A2 protein. J Mol Med (Berl) 99:1115-1123 (2021). PubMed: 33904933

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1-HRP #ab99774
mouse mAb anti human IgG2-HRP #ab99784
mouse mAb anti human IgG3-HRP #ab99829
mouse mAb anti human IgG4-HRP #ab99817

2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

non-pre-adsorbedvs preadsorbed
anti IgM ab97230vs ab98679
anti IgG1 ab98693vsab97240
anti IgG2a ab97245 vs ab98698
anti IgG2b ab97250 vs ab98703
anti IgG2c ab97255 vs ab98722
anti IgG3 ab97260vsab98708


3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

Many thanks,

Tapan Bhattacharyya
London School of Hygiene & Tropical Medicine

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Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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Question

Dear Thank you for your reply. I would like to order mouse secondary IgM antibody that is specific only to IgM and not other immunoglobulins.  I would like to first start with western blotting as I have measured the bands for IgG and IgE and I will do ELISA later after detecting any change in the western blot.  Thank you for your assistance! Best regards,

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Answer

Thank you for your reply. Both antibodies suggested in my last email (ab98671 and ab98743) that were suitable for ELISA should also detect IgM in denatured or reduced forms when being used as primary antibody in WB. We also offer ab98679 which is HRP labelled and could be used directly without need for a secondary antibody that then is HRP labelled. In this case it is not necessary to use an F(ab’)2 antibody. I hope this information was helpful. Please do not hesitate to contact me again with any further questions.

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