Goat Anti-Mouse IgM mu chain (HRP) preadsorbed (ab98679)

Overview

  • Product name

    Goat Anti-Mouse IgM mu chain (HRP) preadsorbed
    See all IgM secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP), pre-adsorbed
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: ICC, WB, ELISA, IHC-Pmore details
  • Minimal
    cross-reactivity


    Human, Rat more details
  • Conjugation

    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab98679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000. Chemiluminescent: 1/5000 - 1/25000).
ELISA 1/10000 - 1/100000. (as Primary).
IHC-P 1/200 - 1/2000.

References

ab98679 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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Answer

Thank you for your reply. Both antibodies suggested in my last email (ab98671 and ab98743) that were suitable for ELISA should also detect IgM in denatured or reduced forms when being used as primary antibody in WB. We also offer ab98679 which is HRP labelled and could be used directly without need for a secondary antibody that then is HRP labelled. In this case it is not necessary to use an F(ab’)2 antibody. I hope this information was helpful. Please do not hesitate to contact me again with any further questions.

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