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Dear technical team, Our customer has ab21035(IgM), ab97230(IgG) and is going to conjugate FITC. She wants to know that antibody has -COOH or -NH2. I know generally whole antibodies have -cooH group at the Fc region but don't know about -NH2. Check this for our customer.
Asked on Dec 13 2011
Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids which have carboxy and amine groups in their side-chains, such as Arginine, Lysine, Glutamate or Aspartate. Therefore, the coupling reaction with FITC that usually takes place via free amine groups forming a stable thiourea bond (as for example with our EasyLink kits) is absolutely feasible for the anti-Enrofloxacin/Ciprofloxacin antibody ab21035. However, as the Goat polyclonal Secondary antibody ab97230 is already conjugated to HRP, I would not recommend to label this antibody with FITC. As alternative, I would suggest using an unconjugated antibody such as ab98671: Click here (or use the following: https://www.abcam.com/index.html?datasheet=98671). We also have a Goat polyclonal Secondary antibody to Mouse IgM (mu chain) available that is already FITC-conjugated (ab97229): Click here (or use the following: https://www.abcam.com/index.html?datasheet=97229). I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.
Answered on Dec 13 2011