Overview

  • Product name
    Goat Anti-Mouse Anti-Rabbit IgG/IgM H&L (HRP polymer)
    See all IgG + IgM secondary antibodies
  • Description
    Goat Anti-Mouse Anti-Rabbit IgG/IgM H&L (HRP polymer)
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    This antibody reacts with heavy and light chains on mouse and rabbit IgG. It reacts with all mouse and rabbit IgG subclasses and with mouse and rabbit IgM.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Conjugation
    HRP polymer

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    PBS pH7.3 with protein carrier and preservative
  • Concentration information loading...
  • Conjugation notes
    HRP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The effect is multiple HRP molecules linked to one single antibody.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes


    This new and innovative HRP-polymerization technology provides a significant increase in staining sensitivity when compared to other conventional HRP-conjugated secondary antibodies. Avidin-biotin blocking procedures are not necessary when using this product. The overall staining procedure uses one less reagent (streptavidin peroxidase) and rinsing step, eliminates avidin-biotin blocking procedures and may reduce overall staining time.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. This product is provided in a pre-diluted, ready-to-use format. Apply for 20 - 30 minutes.

References

This product has been referenced in:
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

Thank you very much for contacting us with your question.
Ab2891 is a prediluted antibody, and it is intended to be used without further dilution, on both frozen and paraffin sections. We don't have larger quantities at this time, but if you need 60 mL or more (10+ bottles), we can arrange a bulk order for you. Please contact sales@abcam.com if this is the case.
I hope this information will be useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

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Question
Answer

Thank you for contacting us.

The expiry date would be 1 year i.e. untill 31st July 2013.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Ihave have received another replyfrom the supplying lab, with a few more details for question 2) especially:

#1) Detection polymer composition is proprietary and that detail cannot be disclosed.

#2) We have not run our polymer detection side-by-side with a non-polymer HRP detection to allow us to give a quantitative answer. We can say that the polymer detection affords a significant increase in sensitivity that will allow users to increase primary antibody titers and/or decrease incubation times.

I hope this information is of some more help.Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.
I have heard back from the supplying lab:

Unfortunately, the answer to the first question (number of HRP molecules per polymer) is proprietary.

As for the secondquestion (% increase in signal intensity compared to regular HRP-secondary antibody): The answer would be subject tomany varying conditions, such asfixation, signal in tissue, section of tissue, HIER, etc. We do not have a general answer that applies to these circumstances in this case.

I am very sorry that I do not have more detailed information which I can give you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. Unfortunately, we are not sure if this product contains the Fc fragment.

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Answer

Thank you for your enquiry. Unfortunately since it has not been previously tested in Western blot and since we do not know the concentration of the secondary antibody (this information is considered confidential by the antibody originator), I can only make a guess at the dilution you would need to use. You may wish to try a few different dilution, I might try a range from 1/10 to 1/100 to begin with, and then see what the staining looks like and adjust the dilutions accordingly. Please do let us know how you make out with this antibody in Western blot, as we would love to know whether it will work in this application and what dilution can be used. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and your patience. This antibody is a polymer detection for mouse and rabbit antibodies on human tissue. It will cross-react with virtually all monoclonals and rabbits antibodies. Specific cross-reactivity with a particular protein has not been observed. As I mentioned on the telephone, the concentration, protein carrier and preservative are proprietary. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. Further to correspondence with the source of this antibody unfortunately I have been unable to determine a precise concentration. They are unable to disclose the antibody concentrations of their detection systems. I can however tell you that this product is provided in a pre-diluted, ready-to-use format. I am sorry that I cannot be of more assistance.

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Answer

I am terribly sorry for the delay in finding the answer to your question regarding the concentration of ab2891. Unfortunately the originator of this antibody cannot disclose this information to us as it is proprietary and must remain confidential. I apologise for the inconvenience,

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Question
Answer

This product is provided in a pre-diluted, ready-to-use format for immunostaining procedures. There is no need to dilute further this secondary antibody. Please refer to our IHC protocols for more information, and if you have any further questions, please do let us know.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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