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Goat Anti-Pig IgG Fc (ab112637)

Submit a review Q&A (2)

Key features and details

  • Goat Anti-Pig IgG Fc
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, WB, ELISA, IHC-P

Conjugates logo Related conjugates and formulations

FITC HRP

Overview

  • Product name

    Goat Anti-Pig IgG Fc
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Pig

  • Specificity

    By immunoelectrophoresis and ELISA ab112637 reacts specifically with Pig IgG. ab112637 may cross react with IgG from other species.

  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. ab112637 was isolated by affinity chromatography using antigen coupled to agarose beads.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab112637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/200 - 1/2000.
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
IHC-P
1/200 - 1/2000.
Notes
ICC/IF
1/200 - 1/2000.
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
IHC-P
1/200 - 1/2000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab112637? Please let us know so that we can cite the reference in this datasheet.

ab112637 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Dear xxxxx, thanks,

that is very helpful. I note your comment on coating, years of experience suggests that a reasonable amount of carrier protein in coating is a preferred option hence my reluctance to use IA purified IgG on solid phases so addition of a touch of homologous serum albumen will do the trick. I assume you have stocks? Will get back to you shortly, Many Thanks

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Answer

Thank you for your reply.

I am sorry but we are not able to supply either the Goat polyclonal Secondary Antibody to Pig IgM(ab112099) or Goat polyclonal Secondary Antibody to Pig IgG - Fc (ab112637) prior to the affinity purification. We do have Goat Serum available from our catalogue which may be of use to you. This is under the catalogue number ab7481.

I hope this information has been of help. Please do not hesitate to contact us again if you have any further questions.

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Question

Good morning.

I wonder if you could help with information, I need to make ELISA wells coated with antibody to Pig gamma-Fc and separately to mu-Fc. These will be used for G and M capture ELISAs. Your product ab112637 is affinity purified and I assume adsorbed for class specificity, ie will not capture IgM antibody via H/L chain reactivity? Please can you confirm this?

The next question relates to ab112099 where the question is essentially the same, is thisknown to be mu-chain Fc specific?

Finally could you supply adsorbed serum prior to immune affinity purification? Coating a solid phase with ia-purified antibody in my experience is often not as good as using a high titre globulin cut from a high titre serum. Grateful for an early response or it might be better if I called you later this morning.

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Answer

Thank you for contacting us and your interest in our products.

I can share the following information in regards to the Goat polyclonal Secondary Antibody to Pig IgM (ab112099) and Goat polyclonal Secondary Antibody to Pig IgG - Fc (ab112637) which may be of help to you.

Yes, theab112099 antiserum has been solid phase adsorbed to ensure class specificity. By immunoelectrophoresis and ELISA this antibody reacts specifically with pig IgM.This antibody may however cross react with IgM from other species.

Similarly, theab112637 antibody is mu-chain Fc specific.

Unfortunately, we are unable to supply you adsorbed serum prior to affinity purification.We would notrecommend coating plates with adsorbed serum prior to affinity purification.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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