Overview

  • Product name
    Goat Anti-Pig IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Pig
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Full length native Swine IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab6911 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you very much for your inquiry and your interest in our products.

    If performing a double or triple staining, it is very important to make all the controls that the secondary antibodies do not crossreact with the immunoglobulins from the other species. For example for the anti pig antibody, it would be important that the anti pig antibody does not recognise the immunoglobulins from mouse or rabbit, not to have a false collocatisation. Unfortunately, non of our anti pig antibodies have been tested for this. However as you have used the ab6775 already successfully in your laboratory if I understood correctly, I can recommend to use the ab6773 as it is from the same original pool. Please do however perform the single color controls to verify the crossreactivity of the ab6773 with rabbit or mouse immunoglobulins.

    In regards to the anti rabbit antibody, I can recommend to use the ab98500, a donkey anti rabbit IgG which has been preabsorbed against mouse, rat and human immunoglobulins, sothere is nocrossreaction against those immunoglobulins. We do not have an anti rabbit DyLight 594 which has been crossabsorbed also against the pig immunoglobulins. If you are concerned or if you have troubles with background staining on the VERO cells, I can recommend to use the same antibody , however with no Fc region, i.e. the F(ab')2 portion. This would be the ab98490.
    https://www.abcam.com/index.html?datasheet=98500 (or use the following: https://www.abcam.com/index.html?datasheet=98500).
    https://www.abcam.com/index.html?datasheet=98490 (or use the following: https://www.abcam.com/index.html?datasheet=98490).
    Please let us know which one you would prefer, so we can send the correctproforma invoice.

    The species in which the secondary antibody is generated is not very important. It is only important if you get background staining from the secondary antibody alone, in which case you can then block with serum of that species. However this is not an issue in general, especially not in cell culture.

    I hope this information is helpful.

    We will send theproforma invoices tomorrow also to your email address. I hope this is how you would like to receive it. Please let me know however whether you would like us to send it to another email address or fax.

    Thank you very much. Please do not hesitate to contact us for any further question or advice.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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