Goat Anti-Pig IgG H&L (HRP) (ab6915)

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Overview

  • Product name

    Goat Anti-Pig IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Pig

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details

  • Immunogen

    Full length native Swine IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
WB
ELISA
Immunomicroscopy
Dot blot
IHC-Fr
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/5000 - 1/20000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Images

    • Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)
      Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)
      Goat Anti-Pig IgG H&L (HRP) (ab6915) at 1/1000 dilution + Pig IgG, 50 ng


      ab6915 incubated for 1 hour at room temperature.

      Both the heavy and light chains of pig IgG are detected.

    References

    This product has been referenced in:

    • Chang YF  et al. Differences in immune responses of pigs vaccinated with Salmonella Typhimurium and S. Choleraesuis strains and challenged with S. Choleraesuis. Comp Immunol Microbiol Infect Dis 65:41-47 (2019). Read more (PubMed: 31300124) »
    • Endmann A  et al. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One 9:e101715 (2014). Read more (PubMed: 24992038) »
    See all 2 Publications for this product

    Publishing research using ab6915? Please let us know so that we can cite the reference in this datasheet.

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