Goat Anti-Pig IgG H&L (HRP) (ab6915)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameGoat Anti-Pig IgG H&L (HRP)
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesPig
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Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
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Immunogen
Full length native Swine IgG (purified).
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ConjugationHRP
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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Conjugation notesHorseradish Peroxidase (HRP)
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab6915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IHC-P | ||
| WB | ||
| ELISA | ||
| Immunomicroscopy | ||
| Dot blot | ||
| IHC-Fr |
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/5000 - 1/20000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/5000 - 1/20000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Images
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Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)Goat Anti-Pig IgG H&L (HRP) (ab6915) at 1/1000 dilution + Pig IgG, 50 ngab6915 incubated for 1 hour at room temperature.
Both the heavy and light chains of pig IgG are detected.
Datasheets and documents
References
This product has been referenced in:
- Endmann A et al. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One 9:e101715 (2014). Read more (PubMed: 24992038) »
