Goat Anti-Pig IgG H&L (HRP) (ab6915)

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Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)

    Key features and details

    • Goat Anti-Pig IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr

    Overview

    • Product name

      Goat Anti-Pig IgG H&L (HRP)
      See all IgG secondary antibodies

    • Host species

      Goat

    • Target species

      Pig

    • Tested applications

      Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details

    • Immunogen

      Full length native Swine IgG (purified).

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

    • Conjugation notes

      Horseradish Peroxidase (HRP)

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6915 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    IHC-P
    WB
    ELISA
    Immunomicroscopy
    Dot blot
    IHC-Fr
    Notes
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/5000 - 1/20000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Images

    • Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)
      Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)
      Goat Anti-Pig IgG H&L (HRP) (ab6915) at 1/1000 dilution + Pig IgG, 50 ng


      ab6915 incubated for 1 hour at room temperature.

      Both the heavy and light chains of pig IgG are detected.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab6915? Please let us know so that we can cite the reference in this datasheet.

    ab6915 has been referenced in 2 publications.

    • Chang YF  et al. Differences in immune responses of pigs vaccinated with Salmonella Typhimurium and S. Choleraesuis strains and challenged with S. Choleraesuis. Comp Immunol Microbiol Infect Dis 65:41-47 (2019). PubMed: 31300124
    • Endmann A  et al. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One 9:e101715 (2014). PubMed: 24992038

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