Goat Anti-Pig IgG H&L (HRP) (ab6915)
Key features and details
- Goat Anti-Pig IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Goat Anti-Pig IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Pig -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
Immunogen
Full length native Swine IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IHC-P | ||
| WB | ||
| ELISA | ||
| Immunomicroscopy | ||
| Dot blot | ||
| IHC-Fr |
| Notes |
|---|
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/5000 - 1/20000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/5000 - 1/20000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Images
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Western blot - Goat Anti-Pig IgG H&L (HRP) (ab6915)Goat Anti-Pig IgG H&L (HRP) (ab6915) at 1/1000 dilution + Pig IgG, 50 ng
ab6915 incubated for 1 hour at room temperature.
Both the heavy and light chains of pig IgG are detected.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab6915 has been referenced in 2 publications.
- Chang YF et al. Differences in immune responses of pigs vaccinated with Salmonella Typhimurium and S. Choleraesuis strains and challenged with S. Choleraesuis. Comp Immunol Microbiol Infect Dis 65:41-47 (2019). PubMed: 31300124
- Endmann A et al. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One 9:e101715 (2014). PubMed: 24992038
