Storage instructionsShipped at Room Temperature. Store at +4°C.
Storage bufferConstituents: Tris buffered saline, 0.1% Tween
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At the core of this product is InnovaCoat® GOLD, a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5 M NaOH at 70°C for >1 hour).
The Goat Anti-Rabbit IgG antibody (GAR) is irreversibly attached to the surface coat via a covalent bond. This irreversible attachment doesn’t allow the desorption of the antibody, which is a major problem with products in which the antibody is only passively adsorbed to the metal.
Goat Anti-Rabbit IgG (80nm Gold, 10 OD) are shipped in a buffer composed of TBS containing 0.1% detergent.
If you wish to exchange the Goat Anti-Rabbit IgG (80nm Gold, 10 OD) into a specific buffer for your assay or test, centrifuge the conjugate in a microfuge at 9,000 x g 6 minutes.
Carefully remove the supernatant, gently tap the pellet and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
If an incubation of Goat Anti-Rabbit IgG (80nm Gold, 10 OD) with the target antibody is performed, do not centrifuge the gold complex after this stage (an antibody titration is recommended).
This product is manufactured by Expedeon, an Abcam company. It was previously called 80nm InnovaCoat® GOLD (10 OD) Goat Anti Rabbit IgG Conjugate. Expedeon product code 221-0200 is the same as the 200 µl size of this product and code 221-1000 is the same as the 1 mL size.
Our Abpromise guarantee covers the use of ab270700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270700 has not yet been referenced specifically in any publications.