Question (29464) | Goat Anti-Rabbit IgG Fc (DyLight® 488) (ab96983)

Go to datasheet (ab96983)

Question

P {margin-top:0;margin-bottom:0;} I am currently using the Alexafluor antibodies from Molecular Probes, but I am willing to try the Dye Light from Abcam. I saw that there were 2 different Goat anti Rabbit Dye Light 488 antibodies on your website. One was pre-adsorbed. What is the difference between those two antibodies and what are the advantages for immunofluorescence experiments? The catalogue numbers are: ab 96983 and ab 98462  

Answer

Thank you for your inquiry. The antibodies ab96983 and ab98462 are essentially the same except that ab98462 is pre-adsorbed.  This means that ab98462 has been pretreated with the normal sera of human, mouse and rat to remove any cross-reactivity with these species.  This is important for customers who are doing multiple staining and worried about cross-reactivity with other species. The advantages of rhe DyLights includes photostability and narrow emission spectra. https://www.abcam.com/index.html?pageconfig=resource&rid=12934#2 I hope this information helps.  Please contact us with any other questions.

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