Product nameGoat Anti-Rabbit IgG H&L (10nm Gold) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: Electron Microscopymore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full lenght rabbit IgG (H and L).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8.20
Preservative: 0.097% Sodium azide
Constituents: Tris buffered saline, 20% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Electron Microscopy||Use at an assay dependent dilution.|
Double immuno-labeling of UGT8 and ceramide in stably transfected PC-3 (Human prostate adenocarcinoma cell line) cells.
Two different secondary antibodies conjugated to different sizes of gold nano-particles (ab105277; 12 nm, labels ceramide, red arrows) and (ab27234; 10 nm, labels UGT8, yellow open arrows). More ceramide labeling and less UGT8 labeling is observed in sham and down regulation samples and the opposite is observed in the up-regulation samples. UGT8 labeling was observed in both the Golgi and the endoplasmic reticulum; both are sites for ceramide metabolism.
Incubated with secondary antibodies at a 1/50 dilution for 45 mins at room temperature.
This product has been referenced in:
- Smith SM et al. TatA complexes exhibit a marked change in organisation in response to expression of the TatBC complex. Biochem J 474:1495-1508 (2017). Electron Microscopy . Read more (PubMed: 28280110) »
- Al-Mahrouki A et al. Microbubble-based enhancement of radiation effect: Role of cell membrane ceramide metabolism. PLoS One 12:e0181951 (2017). Read more (PubMed: 28746357) »