Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (15nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Rabbit IgG

  • Conjugation
    Gold 15nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.20
    Preservative: 0.097% Sodium azide
    Constituents: Tris buffered saline, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:
    • Mao Q  et al. Filamentous Structures Induced by a Phytoreovirus Mediate Viral Release from Salivary Glands in Its Insect Vector. J Virol 91:N/A (2017). Electron Microscopy . Read more (PubMed: 28381575) »
    • Ma X  et al. Essential role for TrpC5-containing extracellular vesicles in breast cancer with chemotherapeutic resistance. Proc Natl Acad Sci U S A 111:6389-94 (2014). Read more (PubMed: 24733904) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for EM and for human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I am sorry to confirm that in this case, as the antibody was purchased in Feb2007, over 5 years ago,we are regrettably not able to provide our guarantee on this occasion, and it is too long to be able to make an exception in this case. It is possible that over this length of time, this antibody has now degraded and is unfortunately no longer usable.

    Although no longer covered by the guarantee, I wouldbe pleasedto investigate this particular case further for you, and also obtain more information for our quality monitoring records. There may be some tips we can provide in order to help improve the results. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    I would appreciate if you could also provide an image which would help us to assess the results.

    In addition,the datasheet for ab27236recommends to store at +4oC. In this case it is not a problem to freeze the antibody as the buffer contains glycerol, and gold conjugation is not affected by freezing. However, we do recommend that individual storage instructions on the datasheet should be followed for eachindividual productin case there is temperature sensitivity. This will help to ensure that the antibody remains stable and that we can continue to provide our 6 month guarantee

    Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

    Order Details
    Antibody code:

    Lot number:

    Purchase order number
    or preferably Abcam order number:


    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, low signal, non-specific satining etc.)

    Sample (Species/Tissue/Cell Type/Cell Line etc.)


    Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


    Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


    Permeabilization step


    Blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Detection method


    Positive and negative controls used (please specify)


    Optimization attempts (problem solving)
    How many times have you tried the IHC?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No


    What steps have you altered?


    Additional Notes


    We would appreciate if you are also able to provide and image which would help us to assess the results

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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