Overview

  • Product name
    Goat Anti-Rabbit IgG H&L
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    This antibody cocktail reacts with heavy and light chains on rabbit IgG and mouse IgG (reacts with all IgG subtypes).
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICCmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Conjugation notes
    HRP or AP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The end result is two antibody subsets: 1) Multiple HRP molecules linked to goat anti-rabbit antibodies. 2) Multiple AP molecules linked to goat anti-mouse antibodies.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is specially designed for a rapid double stain procedure. The overall staining procedure can be done in 4 steps and in less than two hours. Avidin-biotin blocking procedures are not necessary when using this product. This new and innovative AP and HRP polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP or HRP conjugated secondary antibodies.

    This product is provided in a pre-diluted, ready-to-use format. Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS. Equally suited to both manual and automated immunostaining proceedures. This product has not been tested in any application requiring further dilution of the antibody and therefore it is not recommended for use in such applications.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1.
IHC-Fr 1/1.
ICC 1/1.

References

ab2892 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your enquiry. The TBS buffer than we recommend is a general TBS buffer (pH7.6). Please find below the ingredients for the buffer. TBS 10x (concentrated TBS) - 24.23 g Trizma HCl - 80.06 g NaCl - Mix in 800 ml ultra pure water. - pH to 7.6 with pure HCl. - Top up to 1 L. TBS - For 1 L: 100 ml of TBS 10x + 900 ml ultra pure water Further more information on buffers and stock solutions, please see the pdf file link below. https://www.abcam.com/ps/pdf/protocols/Buffers%20and%20stock%20solutions.pdf If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry. Yes, this can be used to perform single staining. However, I would recommend that your customer uses an HRP or AP conjugated secondary antibody together with a suitable chromogen as this would be a more simplistic approach. For example: Goat anti-rabbit IgG antibody (ab7090). However, I appreciate that we do not sell an AP conjugated anti-mouse secondary and if this was the approach it may be necessary to use this kit for detection purposes.

Read More

Answer

Thank you for your enquiry and your interest in our products. We normally suggest TBS instead of PBS for IHC staining. For details on IHC and antigen retrieval, please click on the Resources section on the top panel of the Abcam homepage, you might find some useful information there. As a general rule, for phosphor antibody we would suggest blocking the non-specific binding sites with 1%BSA rather than with milk. We hope this will help.

Read More

Answer

The originator of ab2892 was able to provide me with the following information: mouse (AP)will appear red and rabbit (HRP) will appear brown.

Read More

Answer

Sorry but we do not have a protocol for double staining although we do plan to create a general double staining protocol in the next few months. Please keep an eye on the protocol section on the Abcam website for details.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up