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Thanks for reading this message. I am interested in knowing the reason on why you suggested to use TBS instead of PBS in the application note for catalog# ab2892 (mouse + rabbit double staining secondary antibody). Do you typically get better results with TBS as compared to PBS? If we are using a phosphor antibody, would you recommend using TBS for both the primary and the secondary antibody stain?
Asked on Dec 20 2004
Thank you for your enquiry and your interest in our products. We normally suggest TBS instead of PBS for IHC staining. For details on IHC and antigen retrieval, please click on the Resources section on the top panel of the Abcam homepage, you might find some useful information there. As a general rule, for phosphor antibody we would suggest blocking the non-specific binding sites with 1%BSA rather than with milk. We hope this will help.
Answered on Dec 20 2004