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How many pH of TBS recommed to used in ab2892 procudure? Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS.
Asked on Jul 31 2008
Thank you for your enquiry. The TBS buffer than we recommend is a general TBS buffer (pH7.6). Please find below the ingredients for the buffer. TBS 10x (concentrated TBS) - 24.23 g Trizma HCl - 80.06 g NaCl - Mix in 800 ml ultra pure water. - pH to 7.6 with pure HCl. - Top up to 1 L. TBS - For 1 L: 100 ml of TBS 10x + 900 ml ultra pure water Further more information on buffers and stock solutions, please see the pdf file link below. https://www.abcam.com/ps/pdf/protocols/Buffers%20and%20stock%20solutions.pdf If there is anything else that I can help you with, please do not hesitate to contact me.
Answered on Jul 31 2008