For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    goat-rabbit-igg-hl-alexa-fluor-405-ab175652.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (11)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

Key features and details

  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ELISA, Flow Cyt, IHC-P, ICC/IF

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated Unconjugated

You may also be interested in

Primary
Product image
Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
Conjugation
Product image
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)
Blocking
Product image
Normal Goat Serum (ab7481)

View more associated products

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ELISA, Flow Cyt, IHC-P, ICC/IFmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 405

Associated products

  • Alternative Versions

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
    • Goat Anti-Rabbit IgG H&L (ab182016)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt (1)
1/2000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

Notes
IHC-Fr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 4% PFA fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

    Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

    This data was developed using the unconjugated antibody (ab182016).

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

    Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182016).

  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (11)

Publishing research using ab175652? Please let us know so that we can cite the reference in this datasheet.

ab175652 has been referenced in 11 publications.

  • Harkness JH  et al. Diurnal changes in perineuronal nets and parvalbumin neurons in the rat medial prefrontal cortex. Brain Struct Funct 226:1135-1153 (2021). PubMed: 33585984
  • Kim H  et al. Activation of the Akt1-CREB pathway promotes RNF146 expression to inhibit PARP1-mediated neuronal death. Sci Signal 13:N/A (2020). PubMed: 33443209
  • Madeira N  et al. Temporal Gating of Synaptic Competition in the Amygdala by Cannabinoid Receptor Activation. Cereb Cortex 30:4064-4075 (2020). PubMed: 32163129
  • Mukherjee RN & Levy DL Reticulon 4a promotes exocytosis in mammalian cells. Mol Biol Cell 30:2349-2357 (2019). PubMed: 31318320
  • Kroon T  et al. Early postnatal development of pyramidal neurons across layers of the mouse medial prefrontal cortex. Sci Rep 9:5037 (2019). PubMed: 30911152
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Good Goat Anti-rabbit secondary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample Mouse Brain

Used to detect Abcam Rabbit anti-TMEM19 in primary microglia isolated from mouse brain using image stream flowcytometry.


Other product details

Dilution

1/300

Incubation time:

2 hours Temperature: 25°C

Abcam user community

Verified customer

Submitted May 06 2021

CART in porcine jejunum

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Rabbit antibodies raised against coccaine- and amphetamine-regulated transcipt (CART) were visualised with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) and viewed under an epifluorescent micropsope eqipped with UV filter.

PROF. Marcin Arciszewski

Verified customer

Submitted Mar 31 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.