Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameGoat Anti-Rabbit IgG H&L (Alexa Fluor® 405)
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesRabbit
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SpecificityThis antibody is specific to Rabbit IgG
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Tested applicationsSuitable for: IHC-Fr, ELISA, Flow Cyt, IHC-P, ICC/IFmore details
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Immunogen
The details of the immunogen for this antibody are not available.
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ConjugationAlexa Fluor® 405. Ex: 402nm, Em: 421nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
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ClonalityPolyclonal
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IsotypeIgG
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General notes
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com
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Research areas
Applications
Our Abpromise guarantee covers the use of ab175652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | 1/2000. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)ICC/IF image of ab6046 stained HeLa cells. The cells were 4% PFA fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
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Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
References
This product has been referenced in:
- Tippimanchai DD et al. Adenoviral vectors transduce alveolar macrophages in lung cancer models. Oncoimmunology 7:e1438105 (2018). Read more (PubMed: 29872579) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"