Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    This antibody is specific to Rabbit IgG
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Frmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • ICC/IF image of beta Tubulin staining in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150077 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) and (ab16048, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 (red) goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab150077 Alexa Fluor® 488 (green) goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei.

  • IHC - Wholemount of Caenorhabditis elegans larvae labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab5095. The sample was incubated with primary antibody (1/500 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000), was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Overlay histogram showing HeLa cells stained with ab32356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32356, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:
  • Hu Y  et al. Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function. Theranostics 8:169-184 (2018). Read more (PubMed: 29290800) »
  • Sohn EJ MicroRNA 200c-3p regulates autophagy via upregulation of endoplasmic reticulum stress in PC-3 cells . Cancer Cell Int 18:2 (2018). Read more (PubMed: 29308051) »

See all 195 Publications for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Frozen sections)
I tried many different product to get secondary antibody for immunofluorescence staining better and more clear but failed then I bought abcam ab150077 - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488 in kidney cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best secondary antibody to detect immunofluorescence during in vivo studies
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Dr. Shailendra Singh

Verified customer

Submitted Aug 17 2018

Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Been using this for a while. Very happy with the results!
Username

Sebastian Alvarado

Verified customer

Submitted Dec 09 2015

Application
Immunohistochemistry (Frozen sections)
Primary antibody anti-Glycine (ab9442) was used at 1 in 500. Secondary antibody Goat anti-Rabbit (ab150077) was used at 1 in 1000, for 2 hours at room temperature. Following 3 washes there was still strong staining and little background noise.
Username

Abcam user community

Verified customer

Submitted May 23 2014

Application
Immunocytochemistry/ Immunofluorescence
Nice bright, clear staining observed with secondary antibody (ab150077) at a 1:200 dilution. The primary antibody used was to Human Nanog (ab21624).
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Dr. Sarah Ritson

Verified customer

Submitted Oct 07 2013

Application
Immunocytochemistry/ Immunofluorescence
Following fixation in 4% PFA, the cells were assessed for Sall4 expression using 1:100 dilution of the primary antibody (ab29112) in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using goat polyclonal rabbit IgG Alexa 488 (ab150077) at 1:200. The results show that nuclear Sall4 (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here).
Username

Abcam user community

Verified customer

Submitted Aug 21 2013

Application
Immunocytochemistry/ Immunofluorescence
Cells were fixed in 4% PFA, permeabilized using 0.1% Triton X-100, blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes at RT then incubated with ab124297 at a 1/100 dilution or Rabbit IgG Isotype control for 2 hours at RT. The secondary used was a Goat polyclonal Secondary Antibody to Rabbit IgG – H&L Alexa Fluor 488 (ab150077) used at 1/200 dilution.
Username

Joe Segal

Verified customer

Submitted Aug 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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