This antibody has been used extensively with a number of different primary antibodies generated in rabbits. It performs extremely well at a 1:200 dilution. Attached is an immunofluorescent image of MeOH-fixed HeLa cells that were immunofluorescently labeled with a Lamin A/C (nuclear membrane) antibody (ab133256). The secondary antibody has been pseudo-colored green in the merged image. DNA was counterstained with DAPI.
Dr. Kirk Mcmanus
Submitted Mar 28 2014
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