Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    This antibody is specific to Rabbit IgG
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ELISA, ICC/IF, IHC-Pmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation
    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab175471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/2000.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200 - 1/2000.
IHC-P Use at an assay dependent concentration.

Images

  • ICC/IF image of ab6046 stained HeLa cells. The cells were 100% Methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175471 Alexa Fluor® 568 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody was ab175471 Alexa Fluor® 568 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green DCS1 (ab138905) was used to stain the cell nuclei (green) at a dilution of 1/500.

     

References

This product has been referenced in:
  • Verharen JPH  et al. A neuronal mechanism underlying decision-making deficits during hyperdopaminergic states. Nat Commun 9:731 (2018). Read more (PubMed: 29467419) »
  • Olsen LC  et al. Evidence for a centrosome-attracting body like structure in germ-soma segregation during early development, in the urochordate Oikopleura dioica. BMC Dev Biol 18:4 (2018). Read more (PubMed: 29486709) »
See all 21 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
1:500 dilution (1h incubation) worked well.

Abcam user community

Verified customer

Submitted Jun 04 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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