Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Application: Immunohistochemistry free floating
Sample: Rat Tissue sections (Adult rat brainstem tissue
Incubation time: Overnight · Temperature: 4°C · Diluent: 0.1% PBS-triton
Dilution:1/500
Additional Notes
30 um sections from PFA-perfusion fixed adult animals were cut on a cryostat and blocked for 1 hour in 2% goat serum. Primary antibody incubation was carried out in 0.3% PBST overnight at 4 degrees.
Sample: Rat Tissue sections (Adult rat brainstem tissue
Incubation time: Overnight · Temperature: 4°C · Diluent: 0.1% PBS-triton
Dilution:1/500
Additional Notes
30 um sections from PFA-perfusion fixed adult animals were cut on a cryostat and blocked for 1 hour in 2% goat serum. Primary antibody incubation was carried out in 0.3% PBST overnight at 4 degrees.
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Submitted Jul 22 2021