Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175733)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameGoat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesRabbit
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Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.
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Tested applicationsSuitable for: IHC-Fr, WB, ELISA, ICC/IF, Flow Cyt, IHC-Pmore details
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Minimal
cross-reactivity
Human, Mouse, Rat more details -
Immunogen
The details of the immunogen for this antibody are not available.
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ConjugationAlexa Fluor® 750. Ex: 749nm, Em: 775nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAntiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
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ClonalityPolyclonal
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab175733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-Fr | Use at an assay dependent concentration. This applicaiton is no longer batch tested |
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| WB | 1/10000. | |
| ELISA | Use at an assay dependent concentration. This applicaiton is no longer batch tested |
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| ICC/IF | 1/200 - 1/1000. This applicaiton is no longer batch tested |
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| Flow Cyt | 1/2000. This applicaiton is no longer batch tested |
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| IHC-P | Use at an assay dependent concentration. This applicaiton is no longer batch tested |
Images
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Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175733)Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175733) at 1/10000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175733 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175733)ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab175733 Alexa Fluor® 750 goat anti-rabbit IgG (H+L) preadsorbed used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175733)
References
This product has been referenced in:
- Hu W et al. The complex of TRIP-Br1 and XIAP ubiquitinates and degrades multiple adenylyl cyclase isoforms. Elife 6:N/A (2017). ICC/IF . Read more (PubMed: 28656888) »
