Product nameGoat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details
Rabbit IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|WB||1/1000 - 1/4000.|
|ELISA||1/3000 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|ICC/IF||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
Dot Blot of ab6722.
Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Lane 1: Rabbit IgG.
Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
This product has been referenced in:
- Subramanian K et al. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat Microbiol 4:62-70 (2019). Read more (PubMed: 30420782) »
- Wang Y et al. Electroacupuncture treatment upregulates a7nAChR and inhibits JAK2/STAT3 in dorsal root ganglion of rat with spared nerve injury. J Pain Res 12:1947-1955 (2019). Read more (PubMed: 31308727) »