Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICC
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent dilution.
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WB |
1/1000 - 1/4000.
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ELISA |
1/3000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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ICC/IF |
1/200 - 1/1000.
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IHC-Fr |
Use at an assay dependent dilution.
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ICC |
Use at an assay dependent dilution.
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Notes |
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IHC-P
Use at an assay dependent dilution. |
WB
1/1000 - 1/4000. |
ELISA
1/3000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
ICC/IF
1/200 - 1/1000. |
IHC-Fr
Use at an assay dependent dilution. |
ICC
Use at an assay dependent dilution. |
Images
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Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
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Dot Blot of ab6722.
Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
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Lane 1: Rabbit IgG.
Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
References (27)
ab6722 has been referenced in 27 publications.
- De Clercq K et al. Double-label immunohistochemistry to assess labyrinth structure of the mouse placenta with stereology. Placenta 94:44-47 (2020). PubMed: 32421534
- Jayamohan S et al. Dysregulation of miR-375/AEG-1 Axis by Human Papillomavirus 16/18-E6/E7 Promotes Cellular Proliferation, Migration, and Invasion in Cervical Cancer. Front Oncol 9:847 (2019). PubMed: 31552174
- Kannan M et al. AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 31698701
- Subramanian K et al. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat Microbiol 4:62-70 (2019). PubMed: 30420782
- Wang Y et al. Electroacupuncture treatment upregulates a7nAChR and inhibits JAK2/STAT3 in dorsal root ganglion of rat with spared nerve injury. J Pain Res 12:1947-1955 (2019). PubMed: 31308727