Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

Submit an Abreview Q&A (6)Specific References (15)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
WB 1/1000 - 1/4000.
ELISA 1/3000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
IHC-Fr Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Images

  • Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)
    Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)
    Ab6722 was used at dilution 1/10000 with the primary antibody ab16731 in WB. See the review on ab16731.
  • ELISA - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)
    ELISA - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)
    Ab6722 was used with the primary antibody ab2594 in ELISA. See Abreview on ab2594.

References

This product has been referenced in:
  • Jang J  et al. Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens. Sci Rep 8:7211 (2018). Read more (PubMed: 29739951) »
  • Liu CT  et al. Production of interleukin-4 in CD133+ cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth. Mol Med Rep 13:5068-76 (2016). WB . Read more (PubMed: 27121303) »

See all 15 Publications for this product

Publishing research using ab6722? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

6 reviews or Q&A

10
Votes: Highest First

Do I need to add a stop solution (ie NaOH) at the end of the sandwich ELISA after the colour develops with ALP chromogen or that is not necessary?

Thank you for contacting us.

Just as with a common indirect ELISA it is important to stop the reaction using the appropriate stop solution.

I hope this information is helpful to you. Please do not hesitate to contact us if you...

Read More

I repeated the experiment and diluted plasma samples out to 1:4800 fold but this dilution dependent signal increase phenomenon persisted. In other words, the signal from the 1:4800 fold dilution was higher than the 1:2 dilution! I really doubt if this ...

Read More

I am not aware of any cross reactivity of this antibody with human Ig. However we do offer a number of secondary antibodies which are designed to reduce background levels by either being pre-absorbed against other proteins and Igs or by digestion into ...

Read More

Many thanks for your reply. I really appreciate your suggestions. My detection is working well now. However I am facing some strange results which I would love your thoughts on please:   I am struggling to interpret an indirect ELISA I am making. It ...

Read More

Thank you for contacting me again. It is always a pleasure to hear from you.

After reviewing your email, I am lead to believe that this really is a hook effect as you had mentioned. I am not an expert on this hook effect in plasma specifical...

Read More

Thanks for your advice and suggestions last time. I got another ab7468 and it is working well, both for WB and my reattempt ELISA.   However I have a question which I hope you may have an answer to:   After adding the pNPP substrate, I waited for 3...

Read More

Thank you for contacting us, I am glad to hear that the new product is working well for you.

Usually when color is developing slowly it is one of three issues. 1) the plates and reagents have not come to room temperature. 2) the conjugates a...

Read More

I recently purchased your ab (ie Rabbit anti-human FNDC5 IgG), to be used in a ELISA. I am wondering what detection system would you recommend?   Ie According to the Abcam ELISA protocol, one option is HRP.   So Do I add a secondary ab conjugated (...

Read More

Thank you for contacting Abcam.

Enzymatic detection with an antibody that has been conjugated to HRP or to AP is a widely used and robust method. As this target is located on the peroxisome membrane I would recommend using anAlkaline Phospha...

Read More

I would like to receive a vial of ab34767 as a refund for my order for ab28664. Also, this is my purchasing code for another antibody I have ordered and that does not work with the replacement product you will send me :Po-IG/101857, order on the 18th A...

Read More

Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Cu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up