Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)
- Datasheet
- Specific References (41)
- Protocols
Overview
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Product nameGoat Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr, IHC-Pmore details
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Immunogen
Rabbit IgG whole molecule
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ConjugationBiotin
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. | |
| ELISA | 1/50000 - 1/200000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent dilution. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)This image is courtesy of an anonymous Abreview.ab21156 staining pancreatic amylase in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG H&L (ab6720) (1/2000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG antibody - H&L (ab6720)This image is courtesy of an anonymous Abreviewab28829 (Rabbit polyclonal to STAT6 (phospho Y641)) at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody. -
Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) (ab6720)
References
This product has been referenced in:
- Benskey MJ et al. Silencing Alpha Synuclein in Mature Nigral Neurons Results in Rapid Neuroinflammation and Subsequent Toxicity. Front Mol Neurosci 11:36 (2018). Read more (PubMed: 29497361) »
- Gao H et al. Triptolide induces autophagy and apoptosis through ERK activation in human breast cancer MCF-7 cells. Exp Ther Med 15:3413-3419 (2018). Read more (PubMed: 29545863) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"