• Product name
    Goat Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr, IHC-Pmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab6720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000.
ELISA 1/50000 - 1/200000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent dilution.


  • IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab21156 staining pancreatic amylase in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG H&L (ab6720) (1/2000) was used as the secondary antibody.

    See Abreview

  • ab28829 (Rabbit polyclonal to STAT6 (phospho Y641)) at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.

    See Abreview

  • Ab6720 was used at dilution 1/100 with the primary antibody ab15000 in IHC-Fr. See the review on ab15000.


This product has been referenced in:
  • Benskey MJ  et al. Silencing Alpha Synuclein in Mature Nigral Neurons Results in Rapid Neuroinflammation and Subsequent Toxicity. Front Mol Neurosci 11:36 (2018). Read more (PubMed: 29497361) »
  • Gao H  et al. Triptolide induces autophagy and apoptosis through ERK activation in human breast cancer MCF-7 cells. Exp Ther Med 15:3413-3419 (2018). Read more (PubMed: 29545863) »

See all 41 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I would be pleased to provide you with the Abtrial testing discount codes. In order to do so, could you please confirm which distributor you will be purchasing the antibodies from?

I look forward to receiving...

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Thank you for contacting us and your interest in our products.

The antibodies you have chosen for your ELISA should work well together. The immunogen used to raise the mouse monoclonal ab54219 does not cover the Y223 region of BTK and they sh...

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Thank you for contacting us and you interest in our products.

There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of...

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Thank you for contacting Abcam.

As you mentioned in your email, we at Abcam cannot guarantee that these antibodies would still work after this time, but if have enough samples to run a few test batches to check the validity of the antibodies, ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has...

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Thank you for your message and for providing this further information.

Thank you for taking the time to provide the additional details to me. These have been of great help in understanding the issue. I am sorry to hear that this product had pe...

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Thank you for contacting us.

I am sorry to hear of the difficulties that you have been experiencing using this product and wish to thank you for performing appropriate steps to determine whether the issue is with the product or protocol.

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Thank you for providing that extra information. It has helped greatly to understand what you have been doing and what may help to improve the signal of the staining. There are a few areas that I would suggest would be worth optimising (if you have not...

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Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully unde...

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Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97...

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1-10 of 13 Abreviews or Q&A


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