Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)
Key features and details
- Goat Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr, IHC-P
Overview
-
Product name
Goat Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr, IHC-Pmore details -
Immunogen
Rabbit IgG whole molecule
-
Conjugation
Biotin
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000 - 1/20000.
|
|
ELISA |
1/50000 - 1/200000.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent dilution.
|
Notes |
---|
WB
1/5000 - 1/20000. |
ELISA
1/50000 - 1/200000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent dilution. |
Images
-
IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
ab6720 was used as secondary antobody at 1/1000 dilution for 1 hour at room temperature to detect FOXL2 in mock -transfected and FOXL2-transfected fibroblasts and glandular epithelial cells in ICC/IF. The primary antibody was a Rabbit Anti-FOXL2.
(From Figure 4B of Lesage-Padilla et al)
-
ab28829 (Rabbit polyclonal to STAT6 (phospho Y641)) at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.
-
ab21156 staining pancreatic amylase in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG H&L (ab6720) (1/2000) was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (164)
ab6720 has been referenced in 164 publications.
- Dicks AR et al. Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells. Methods Mol Biol 2598:87-114 (2023). PubMed: 36355287
- Fuyuki A et al. Expression of prosaposin and its G protein-coupled receptor (GPR) 37 in mouse cochlear and vestibular nuclei. J Vet Med Sci 85:266-270 (2023). PubMed: 36696997
- Costa GMJ et al. High SARS-CoV-2 tropism and activation of immune cells in the testes of non-vaccinated deceased COVID-19 patients. BMC Biol 21:36 (2023). PubMed: 36797789
- Dicks AR et al. Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes. Elife 12:N/A (2023). PubMed: 36810131
- Kasap E et al. The Role of Endoplasmic Reticulum Stress in Gastroesophageal Reflux Disease Symptoms and Treatment. Turk J Gastroenterol 34:533-541 (2023). PubMed: 37158535